So far, the investigation of stem cell aging has been primarily carried out at the genomic level to follow transcriptome changes, clonal dominance, epigenetic changes, and, more recently, population heterogeneity by single cell sequencing. Here, we review recent findings in the field of stem cell aging that include failure of proteostasis, the impact of age-related metabolic changes on stem cell differentiation and heterogeneity of stem cell niches. These emerging concepts highlight the need of a paradigm shift to move forward our understanding of stem cell aging to the next level, in particular, the need of investigating cell intrinsic changes in stem cells and their niche by looking in a quantitative manner to proteins and metabolites.

到目前为止，干细胞衰老的研究主要在基因组水平上进行，以通过单细胞测序跟踪转录组变化、克隆优势、表观遗传变化以及最近的群体异质性。在这里，我们回顾了干细胞衰老领域的最新发现，包括蛋白质稳态失败、与年龄相关的代谢变化对干细胞分化的影响以及干细胞壁龛的异质性。这些新兴概念强调了范式转变的需要，以将我们对干细胞衰老的理解提升到一个新的水平，特别是需要通过定量研究蛋白质和代谢物来研究干细胞及其生态位的细胞内在变化.