Herein, we report the bioactivity of monodispersed nanosized reduced graphene oxide (RGO) enfolded gold nanoparticles (AuNPs) engineered polycaprolactone (PCL) based electrospun composite scaffolds. The 2D patterns of PCL based nanofibers prepared by the homogenous distribution of RGO-AuNPs exhibited unique topological and biological features such as mechanical properties, porous structure, large surface area, high electrical conductivity, biodegradability, and resemble the natural extracellular matrix (ECM) that supports the adhesion, growth, proliferation, and differentiation of stem cells. The prepared composite nanofibers based scaffolds containing RGO-AuNPs accelerated neuronal cell functions and confirmed that the optimized concentration showed cytocompatibility to PC12 and S42 cells. The 0.0005 wt% loading of RGO-AuNPs on PCL has a huge impact on neurite growth which leads to an almost one-fold increase in neurite length growth. The present study provides a new strategic design of highly efficient scaffolds that have a significant direct impact on cell activity and could be a potential bioimplant for peripheral nerve repair.

在这里，我们报告了基于电纺复合支架的单分散纳米尺寸还原氧化石墨烯（RGO）包裹金纳米颗粒（AuNPs）工程聚己内酯（PCL）的生物活性。通过RGO-AuNPs的均匀分布制备的PCL基纳米纤维的2D图样具有独特的拓扑和生物学特征，例如机械性能，多孔结构，大表面积，高电导率，可生物降解性，并且类似于天然的细胞外基质（ECM），支持干细胞的粘附，生长，增殖和分化。制备的包含RGO-AuNPs的基于复合纳米纤维的支架可加速神经元细胞功能，并确认最适浓度显示对PC12和S42细胞具有细胞相容性。0。RPC-AuNPs在PCL上的负载量为0005 wt％，对神经突生长有巨大影响，这导致神经突长度增长几乎增加了一倍。本研究提供了高效支架的新战略设计，该支架对细胞活性具有直接的重大影响，并且可能是潜在的生物植入物，用于修复周围神经。