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De novo transcriptome assembly for Pachygrapsus marmoratus, an intertidal brachyuran crab
Marine Genomics ( IF 1.3 ) Pub Date : 2020-06-22 , DOI: 10.1016/j.margen.2020.100792
Sara Pinosio 1 , Sara Fratini 2 , Stefano Cannicci 3 , Matteo Oliva 4 , Carlo Pretti 5 , Mariella Baratti 6
Affiliation  

The marble crab Pachygrapsus marmoratus inhabits the rocky shores of the Mediterranean Sea, Black Sea and East Atlantic Ocean. As other intertidal species, it is considered a model species to study the effects of environmental stressors on natural populations. In this study, we performed Illumina next-generation sequencing on eleven P. marmoratus specimens with the aims to (i) reconstruct their whole transcriptome, (ii) perform a functional annotation of the assembled transcriptome and (iii) develop gene-based markers for future genetic and genomic studies on this as well as other brachyuran species. We obtained a transcriptome assembly constituted by 56,308 unigenes and covering about 60.3 Mbp. We detected 43,915 Simple Sequence Repeats (SSRs) and 192,631 high-quality Single Nucleotide Polymorphisms (SNPs). Due to the scarcity of genomic resources in decapods, and crabs in particular, our results constitute a valuable resource for future studies on brachyuran crabs. The present data also represent a sound resource to investigate biological responses to pollution in intertidal and marine populations.



中文翻译:

潮间带短尾蟹 Pachygrapsus marmoratus 的从头转录组组装

大理石蟹Pachygrapsus marmoratus栖息在地中海、黑海和东大西洋的岩石海岸。与其他潮间带物种一样,它被认为是研究环境压力源对自然种群影响的模式物种。在本研究中,我们对 11 只P. marmoratus进行了 Illumina 新一代测序标本的目的是 (i) 重建其整个转录组,(ii) 对组装的转录组进行功能注释,以及 (iii) 开发基于基因的标记,用于未来对该物种以及其他短尾鱼物种的遗传和基因组研究。我们获得了由 56,308 个 unigenes 组成的转录组组装,覆盖约 60.3 Mbp。我们检测到 43,915 个简单序列重复 (SSR) 和 192,631 个高质量的单核苷酸多态性 (SNP)。由于十足目动物,尤其是螃蟹的基因组资源稀缺,我们的研究结果构成了未来短尾蟹研究的宝贵资源。目前的数据也代表了研究潮间带和海洋种群对污染的生物反应的可靠资源。

更新日期:2020-06-22
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