Flavonoids are known for their wide range of bioactive properties including beneficial effect on bone formation. Their intense metal ion chelating capacity endorsed their nomination as a new biomaterial for biomedical applications. The present study examined the functional role of Kaemferal-Zinc(II) (Kaem-Zn) complex in bone formation, in vitro and in vivo. The cyto-compatibility assay confirmed that upto 25 μM of Kaem and Kaem-Zn complex was non-toxic. In fact, it facilitates ALP activity and accumulation of calcium in osteoblast; it was confirmed by Alizarin red and von Kossa staining. In addition to this, osteoblast markers, Runx2, type 1 col., ALP mRNAs expression, and osteocalcin and osteonectin secretory proteins level were also induced by the Kaem-Zn complex. Furthermore, bone forming ability of Kaem and Kaem-Zn was assessed by zebrafish model. The optimal concentration of Kaem and Kaem-Zn was determined by the viability assay of Zebrafish larvae. Osteoblasts distribution in scale, vertebrae and caudal fin ossification was studied by alizarin red staining accompanied by confocal imaging were carried out in adult zebrafish exposed to Kaem and Kaem-Zn complex. To sum up, our findings showed that Kaem promotes bone growth, and Kaem-Zn complex has further strengthened it. Kaem-Zn complex could be an effectively explored and used use in bone tissue engineering.

类黄酮因其广泛的生物活性而闻名，其中包括对骨形成的有益作用。它们具有强大的金属离子螯合能力，因此被提名为生物医学应用的新型生物材料。本研究检查了Kaemferal-Zinc（II）（Kaem-Zn）复合物在体外和体内在骨形成中的功能作用。细胞相容性试验证实，高达25μM的Kaem和Kaem-Zn复合物是无毒的。实际上，它促进了ALP活性和成骨细胞中钙的积累。茜素红和von Kossa染色证实了这一点。除此之外，Kaem-Zn复合物还诱导成骨细胞标记，Runx2、1型col。，ALP mRNA表达以及骨钙素和骨连接素分泌蛋白水平。此外，通过斑马鱼模型评估了Kaem和Kaem-Zn的骨形成能力。Kaem和Kaem-Zn的最佳浓度通过斑马鱼幼虫的活力测定来确定。在接触Kaem和Kaem-Zn复合物的成年斑马鱼中，通过茜素红染色和共聚焦成像研究成骨细胞在鳞片，椎骨和尾鳍骨化中的分布。总结一下，我们的发现表明，Kaem促进骨骼生长，而Kaem-Zn复合物进一步增强了骨骼。Kaem-Zn复合物可以在骨组织工程中得到有效的探索和使用。