Synthesis of zeolitic imidazolate framework-8 nanoparticles (ZIF-8 NPs) and evaluation of their potential threats on ecosystem functioning has been reported in this work. A 45-day indoor experiment was conducted to explore the effects of ZIF-8 NPs at three different concentrations (10, 100, and 1000 μg L⁻¹) on the aquatic fungal community associated with Populus nigra L. leaf litter decomposition. After chronic exposure, ZIF-8 NPs at 1000 μg L⁻¹ significantly inhibited fungal biomass and extracellular enzyme activities as a result of inhibition on carbon and nitrogen loss of leaves. Besides, ZIF-8 NPs at 10 μg L⁻¹ increased the percentage of Anguillospora in the fungal community and led Monographella cucumerina and Mycosphaerella tassiana to become the hub species, which eventually significantly promoted the decomposition of leaf litter. In conclusion, our study provides a reference for the possible ecotoxicity of ZIF-8 NPs on aquatic fungi, confirms the influence of ZIF-8 NPs on nutrient cycling in streams, and also emphasizes the importance of fungal community structure and hub species in the process of leaf litter decomposition.

这项工作已报道了合成的沸石咪唑盐骨架8纳米粒子（ZIF-8 NPs）及其对生态系统功能的潜在威胁的评估。进行了为期45天的室内实验，以探讨三种不同浓度（10、100和1000μgL ^-1）的ZIF-8 NP对黑杨叶片凋落物分解相关的水生真菌群落的影响。长期暴露后，由于抑制了叶片的碳和氮损失，1000μgL ^-1的ZIF-8 NPs显着抑制了真菌生物量和细胞外酶的活性。此外，ZIF-8 NPs在10μgL ^-1时增加了真菌群落中gu菌的百分比，并导致黄瓜小单胞菌和塔氏霉菌成为中心物种，最终显着促进了叶片凋落物的分解。总之，我们的研究为ZIF-8 NP对水生真菌的可能生态毒性提供了参考，证实了ZIF-8 NP对溪流养分循环的影响，并且强调了真菌群落结构和枢纽物种在此过程中的重要性。凋落物分解。