Abstract Rumex nepalensis Spreng. (Polygonaceae) is an edible plant rich in anthraquinones, that significantly inhibits Pancreatic Lipase (PL), a key enzyme in the dietary fat digestion. The effectiveness of Ultrasonic Assisted Extraction (UAE) of R.nepalensis was investigated using ethyl acetate as a solvent. Further, optimization of extraction conditions required for maximizing the PL inhibitory activity was determined using response surface methodology with Box-Behnken Design (BBD with three factor, 17 experimental runs). Extraction time (A, min), solid: solvent ratio (B, g/mL) and temperature (C, °C) were selected as the variables. The optimum conditions were established as extraction time (37 min), solid: solvent ratio (1:10.5) and extraction temperature 33 °C. However, till date, no HPTLC reports are available for the simultaneous quantification of chrysophanol and physcion from R. nepalensis. Thus, chrysophanol and physcion were quantified in various extractives of R. nepalensis via. a validated HPTLC method and a positive correlation were obtained with PL inhibition activity (Pearson’s r = 0.801 and 0.755 for chrysophanol and physcion respectively), that justified the PL inhibition potential of R. nepalensis derived anthraquinones.

中文翻译：

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茴香蒽醌提取条件的优化及其与胰脂肪酶抑制活性的相关性

摘要 Rumex nepalensis Spreng。（蓼科）是一种富含蒽醌的可食用植物，可显着抑制胰脂肪酶（PL），这是膳食脂肪消化的关键酶。使用乙酸乙酯作为溶剂研究了超声波辅助提取 (UAE) 的尼泊尔红豆杉的有效性。此外，使用响应面方法和 Box-Behnken 设计（具有三因素的 BBD，17 次实验运行）确定最大化 PL 抑制活性所需的提取条件的优化。选择提取时间 (A, min)、固体:溶剂比 (B, g/mL) 和温度 (C, °C) 作为变量。确定的最佳条件为提取时间（37 分钟）、固溶剂比（1:10.5）和提取温度 33 °C。然而，迄今为止，没有 HPTLC 报告可用于同时定量来自 R. nepalensis 的大黄酚和灵芝。因此，大黄酚和 physcion 在 R. nepalensis 的各种提取物中进行了量化。经验证的 HPTLC 方法和 PL 抑制活性（大黄酚和 physcion 的 Pearson's r 分别为 0.801 和 0.755）获得了正相关，这证明了尼泊尔红藻衍生的蒽醌类的 PL 抑制潜力。