A new type of restricted access media-imprinted nanomaterials (RAM-MIPs) were successfully prepared on the surface of metal-organic framework by reversible addition fragmentation chain transfer polymerization technology. Then it was applied as a dispersed solid phase extraction (DSPE) material in analysis of fluoroquinolones (ofloxacin, pefloxacin, norfloxacin, enrofloxacin and gatifloxacin) in untreated milk and river water by HPLC-UV detection. The resulted material has a good binding amounts (60.81 mg g⁻¹), rapid binding kinetic (15 min) and satisfactory selectivity as well as has a good ability to eliminate matrix interference. Several major factors affecting DSPE efficiency, pH of sample solution, dosage of RAM-MIPs, adsorption time and volume ratios of elution solvent were primarily optimized. In optimization conditions, RAM-MIPs-DSPE was combined with HPLC-UV to enrich fluoroquinolones in untreated milk and river water, achieving satisfactory linear correlation (R² > 0.9988), good limits of detection (LOD, 1.02–3.15 µg L⁻¹ for milk and 0.93–2.87 µg L⁻¹ for river water) and better recoveries (80.7–103.5% and 85.1–105.9% with relative standard deviation (RSD) of not higher than 5.3% and 4.7% for milk and river water samples, respectively). The research results illustrate that it provides a simple and efficient method for the direct detection of FQs in complex samples.

通过可逆加成断裂链转移聚合技术成功地在金属有机骨架表面制备了一种新型的受限访问介质印迹纳米材料（RAM-MIP）。然后将其作为分散固相萃取（DSPE）材料，用于通过HPLC-UV检测分析未经处理的牛奶和河水中的氟喹诺酮类药物（氧氟沙星，培氟沙星，诺氟沙星，恩诺沙星和加替沙星）。所得材料具有良好的结合量（60.81 mg g ^-1），快速的结合动力学（15分钟）和令人满意的选择性以及消除基质干扰的良好能力。主要优化了影响DSPE效率，样品溶液的pH值，RAM-MIP的剂量，吸附时间和洗脱溶剂的体积比的几个主要因素。在优化条件下，RAM-MIPs-DSPE与HPLC-UV结合可在未经处理的牛奶和河水中富集氟喹诺酮类药物，获得令人满意的线性相关性（R ² > 0.9988），检测限良好（LOD，1.02-3.15 µg L ^-1牛奶和0.93–2.87 µg L ^-1河流水）和更好的回收率（牛奶和河流水样品的相对标准偏差（RSD）分别不高于5.3％和4.7％的80.7–103.5％和85.1–105.9％）。研究结果表明，它为直接检测复杂样品中的FQ提供了一种简单有效的方法。