Palm oil mill effluent (POME) was tested as a substrate to produce hydrogen by dark fermentation. Two microbial consortia and a pure culture of Clostridium beijerinckii (ATCC 8260) were cultured anaerobically in raw, diluted and hydrolyzed POME to compare biohydrogen production yields in all three media. Experiments were done in 15 mL Hungate tubes containing 5 mL of medium and 1 mL of inoculum. When Clostridium beijerinckii was cultivated at 30 °C in the hydrolyzed POME (P003), containing 7.5 g/L of sucrose, during 8 days of fermentation and 20 % of the inoculum, the maximum biohydrogen production yield was 4.62 LH₂/L_med. Consortium C3 also showed the best production in hydrolyzed POME while consortium C6 achieved its maximum production in raw POME. This effluent is a potential substrate for biohydrogen production.

棕榈油厂流出物 (POME) 被测试作为通过暗发酵生产氢气的底物。两种微生物聚生体和一种纯培养物拜氏梭菌(ATCC 8260) 在未加工、稀释和水解的 POME 中厌氧培养，以比较所有三种培养基中的生物制氢产量。实验在含有 5 mL 培养基和 1 mL 接种物的 15 mL Hungate 管中进行。当Clostridium beijerinckii在 30 °C 下在含有 7.5 g/L 蔗糖的水解 POME (P003) 中培养 8 天发酵和 20% 的接种物时，最大生物制氢产量为 4.62 LH ₂ /L _med. Consortium C3 在水解 POME 中的产量也最高，而 consortium C6 在原始 POME 中的产量最高。这种流出物是生物制氢的潜在底物。