Reliable and sensitive detection of lipase activity is essential for the early diagnosis and monitoring of acute pancreatitis or progression of digestive diseases. However, the available fluorescent probes for detection of lipase activity are only implemented in a hexane-water two-phase system due to the nature of heterogeneous catalysis of lipase, thus limiting their applications in direct imaging of lipase activity in living cells and tissues. Here we designed and synthesized a “turn on” fluorescent probe CPP based on self-assembled micelles for hydrolysis of lipase. The CPP probe exhibits high selectivity and excellent sensitivity for the detection of lipase in such a homogeneous system and is successfully applied for monitoring lipase activity in pancreatic AR42J cells, tissues, and serums. Taken together, the fluorescent CPP probe not only provides a tool for diagnostic potential in pancreatic disease but also demonstrates an application potential for micelle self-assembly-based development of biological probes.

脂肪酶活性的可靠和灵敏的检测对于急性胰腺炎或消化系统疾病的早期诊断和监测至关重要。然而，由于脂肪酶的非均相催化的性质，用于检测脂肪酶活性的可用荧光探针仅在己烷-水两相系统中实现，因此限制了它们在活细胞和组织中对脂肪酶活性的直接成像中的应用。在这里，我们设计并合成了基于自组装胶束的“开启”荧光探针CPP，用于水解脂肪酶。CPP探针在这种均质系统中对脂肪酶的检测具有很高的选择性和出色的灵敏度，并且已成功应用于监测胰腺AR42J细胞，组织和血清中的脂肪酶活性。在一起