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The Contribution of lincRNAs at the Interface between Cell Cycle Regulation and Cell State Maintenance.
iScience ( IF 4.6 ) Pub Date : 2020-06-20 , DOI: 10.1016/j.isci.2020.101291
Adriano Biasini 1 , Adam Alexander Thil Smith 1 , Baroj Abdulkarim 1 , Maria Ferreira da Silva 1 , Jennifer Yihong Tan 1 , Ana Claudia Marques 1
Affiliation  

Cell cycle progression is controlled by the interplay of established cell cycle regulators. Changes in these regulators' activity underpin differences in cell cycle kinetics between cell types. We investigated whether long intergenic noncoding RNAs (lincRNAs) contribute to embryonic stem cell cycle adaptations. Using single-cell RNA sequencing data for mouse embryonic stem cells (mESCs) staged as G1, S, or G2/M we found differentially expressed lincRNAs are enriched among cell cycle-regulated genes. These lincRNAs (CC-lincRNAs) are co-expressed with genes involved in cell cycle regulation. We tested the impact of two CC-lincRNA candidates and show using CRISPR activation that increasing their expression is associated with deregulated cell cycle progression. Interestingly, CC-lincRNAs are often differentially expressed between G1 and S, their promoters are enriched in pluripotency transcription factor (TF) binding sites, and their transcripts are frequently co-regulated with genes involved in the maintenance of pluripotency, suggesting a contribution of CC-lincRNAs to mESC cell cycle adaptations.



中文翻译:

lincRNA在细胞周期调控和细胞状态维持之间的界面上的贡献。

细胞周期进程由已建立的细胞周期调节因子的相互作用控制。这些调节剂活性的变化是细胞类型之间细胞周期动力学差异的基础。我们调查了长基因间非编码RNA(lincRNA)是否有助于胚胎干细胞周期适应。使用分阶段为G1,S或G2 / M的小鼠胚胎干细胞(mESC)的单细胞RNA测序数据,我们发现差异表达的lincRNA富含细胞周期调控的基因。这些lincRNA(CC-lincRNA)与参与细胞周期调控的基因共表达。我们测试了两种CC-lincRNA候选物的影响,并使用CRISPR激活显示增加其表达与细胞周期进程失调有关。有趣的是,CC-lincRNA通常在G1和S之间差异表达,

更新日期:2020-06-20
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