The mosquito Aedes aegypti L. is a vector transmitting diseases such as dengue, chikungunya and Zika virus fever. The water-soluble lectin from Moringa oleifera Lam. seeds (WSMoL) is larvicidal, ovicidal and can stimulate oviposition in A. aegypti. This study aimed to investigate whether WSMoL could bind to membrane proteins from A. aegypti legs. Initially, proteins from the legs were extracted using sodium deoxycholate, digitonin, dodecyl sodium sulfate (SDS) or Triton X-100. The protein concentration was found to be higher in the extract obtained using Triton X-100, which was applied to a WSMoL-Sepharose column. The adsorbed proteins were evaluated using gel filtration chromatography and polyacrylamide gel electrophoresis (PAGE) in presence of SDS. The similarity in the sequences of adsorbed proteins with those available in databases was determined. The proteins adsorbed on the matrix were eluted forming a single peak. Gel filtration chromatography and SDS-PAGE revealed the presence of proteins with molecular masses of approximately 20 kDa and polypeptide bands of 17.0 and 23.7 kDa, respectively. MS/MS analysis indicated similarity between these proteins and ABC carriers, which are expressed in the legs of mosquitos. WSMoL could bind to membrane proteins in the legs of A. aegypti females and induce oviposition through these interactions.

蚊埃及伊蚊是一种传播疾病的媒介，例如登革热，基孔肯雅热和寨卡病毒热。辣木中的水溶性凝集素。种子（WSMoL）是杀幼虫的，杀卵的，可以刺激埃及伊蚊的产卵。这项研究旨在调查WSMoL是否可以结合埃及伊蚊的膜蛋白腿。最初，使用脱氧胆酸钠，洋地黄皂苷，十二烷基硫酸钠（SDS）或Triton X-100从腿中提取蛋白质。发现使用Triton X-100获得的提取物中的蛋白质浓度更高，将其应用于WSMoL-Sepharose色谱柱。在SDS存在下，使用凝胶过滤色谱法和聚丙烯酰胺凝胶电泳（PAGE）评估吸附的蛋白质。确定了吸附蛋白序列与数据库中可用蛋白的序列相似性。洗脱吸附在基质上的蛋白质，形成一个单峰。凝胶过滤色谱法和SDS-PAGE分析表明存在分子量分别为约20 kDa和多肽条带分别为17.0和23.7 kDa的蛋白质。MS / MS分析表明这些蛋白质与ABC载体相似，表现在蚊子的腿上。WSMoL可以结合到腿的膜蛋白通过这些相互作用，埃及伊蚊和雌性产卵。