In this work, multi-layer wound dressing was made of laminated layers of electrospun fibers supported by adhesive sheet. Graft copolymerization of methyl methacrylate (MMA) and 2-Ethyl-1-hexyl acrylate (EHA) onto carboxymethyl cellulose (CMC) was conducted to obtain an adhesive sheet with 1.52 (N/cm²) loop tack, 1.7 (N/cm) peel strength and 25 s shear strength. Diclofenac sodium, anti-inflammatory drug, was loaded to the adhesive sheet with encapsulation efficiency 73%.

The contact layer to wound was made of synthesized anti-bleeding agents, chitosan iodoacetamide (CI) loaded into electrospun polyvinyl alcohol (PVA) fibers. It was fabricated from fiber diameter 300 nm by electrospinning of 5% wt/v of CI (D.S. 18.7%) mixed with 10% wt/v PVA, at 20 kV and 17 cm airgap. The second, pain-relief layer was fabricated by encapsulating up to 50% wt/wt of capsaicin into gelatin nanofibers (197 nm) crosslinked by glyoxal. The third, antimicrobial layer was fabricated from PVA electrospun fibers loaded with 2% wt/wt gentamicin. Biocompatibility test showed insignificant adverse effects of the fabricated layers on fibroblast cells. Animal test on rat showed accelerated wound healing from 21 to 7 days for the multi-layer dressing. Histopathological findings corroborated the intactness of the epidermis layer of the treated samples.

在这项工作中，多层伤口敷料由粘合片支撑的电纺纤维的层压层制成。进行甲基丙烯酸甲酯（MMA）和丙烯酸2-乙基-1-己酯（EHA）在羧甲基纤维素（CMC）上的接枝共聚，从而获得具有1.52（N / cm ²）循环粘性，1.7（N / cm）的粘合片。剥离强度和25 s剪切强度。将抗炎药双氯芬酸钠以73％的封装效率加载到粘合片上。

所述接触层伤口制成合成的抗出血剂，装入电纺丝聚壳聚糖碘乙酰胺（CI）v inyl醇（PVA）纤维。它是由直径为300 nm的纤维通过5％wt / v的电纺丝制成的的CI（DS 18.7％）与10％wt / v PVA混合，电压为20 kV，气隙为17 cm。第二层止痛层是通过将高达50％wt / wt的辣椒素封装到通过乙二醛交联的明胶纳米纤维（197 nm）中而制成的。第三抗菌层由负载2％wt / wt庆大霉素的PVA电纺纤维制成。生物相容性测试表明，所制造的层对成纤维细胞的不良影响很小。对大鼠的动物试验表明，多层敷料的伤口愈合速度从21天提高到7天。组织病理学结果证实了所处理样品的表皮层的完整性。