The potential of least explored Sorghum durra as a feedstock for enzymatic saccharification in bioethanol production was evaluated as only one report is available. The pretreatment specific, optimization of saccharification of Sorghum durra stalk (Sds) was carried out. The concentrations of crude recombinant endoglucanase (BaGH5-UV2), cellobiohydrolase (CtCBH5A) and β-glucosidase (CtGH1) in cocktail were optimized for saccharification of acid or base pretreated Sds by using mixture design approach. Optimized percentage ratio of BaGH5-UV2, CtCBH5A and CtGH1 with total enzyme loading 2000 U/g of pretreated Sds for acid pretreated Sds was 43.1:10.0:46.9 and for base pretreated Sds was 80:10:10 at 48 h of saccharification. The base pretreated Sds showed 1.8-fold (165.6 mg/g of pretreated Sds) higher total reducing sugar (TRS) yield and 1.2-fold (81.7 mg/g of pretreated Sds) higher glucose yield than acid pretreated Sds. This low sugar yield from acid pretreated Sds was due to the presence of higher acid insoluble lignin content, 11.9 % (w/w) than the base pretreated biomass (2.2 %, w/w). In acid pretreated Sds, ANOVA data showed 3.7-fold higher F value (111.58) for BaGH5-UV2 and CtGH1 interaction than the base pretreated Sds. This indicated that the synergism between BaGH5-UV2 and CtGH1 for acid pretreated Sds was significantly higher. This was due to lower intact hemicellulose content (10.6 %, w/w) in acid pretreated Sds than base pretreated Sds (21.8 %, w/w). The optimized enzyme ratios for saccharification of acid pretreated Sds gave maximum TRS and glucose yield at 72 h, 113 and 85 mg/g of pretreated Sds, respectively, unlike base pretreated Sds at 96 h, 211 and 174 mg/g of pretreated Sds, respectively. These results showed that the ratio of cellulase enzymes in cocktail and their synergism depends on the type of pretreatment used for biomass. Thus, this in-house cocktail design specific to pretreatment can improve the biomass saccharification at large scale.

由于仅有一份报告，评估了最少探索的高粱作为生物乙醇生产中酶促糖化原料的潜力。对预处理进行了优化，优化了高粱茎秆（Sds）的糖化度。使用混合设计方法优化了鸡尾酒中粗制重组内切葡聚糖酶（Ba GH5-UV2），纤维二糖水解酶（Ct CBH5A）和β-葡萄糖苷酶（Ct GH1）的浓度，以糖化酸或碱预处理的Sds。Ba GH5-UV2，Ct CBH5A和Ct的最佳比例在糖化48小时时，总酶负荷GH1为2000 U / g，酸预处理的Sds为43.1：10.0：46.9，碱预处理的Sds为80:10:10。碱预处理的Sds显示的总还原糖（TRS）产量比酸预处理的Sds高1.8倍（165.6 mg / g预处理的Sds）和1.2倍（81.7 mg / g预处理的Sds）高。酸预处理的Sds的这种低糖收率是由于存在比基础预处理的生物质（2.2％，w / w）高的酸不溶性木质素含量11.9％（w / w）。在酸预处理的Sds中，ANOVA数据显示，Ba GH5-UV2和Ct GH1相互作用的F值（111.58）比基础预处理的Sds高3.7倍。这表明Ba GH5-UV2与Ct之间的协同作用酸预处理的Sds的GH1明显更高。这是由于酸预处理的Sds中的完整半纤维素含量（10.6％，w / w）比碱预处理的Sds（21.8％，w / w）低。酸预处理Sds的糖化优化酶比分别在72 h，113和85 mg / g预处理Sds时分别提供了最大的TRS和葡萄糖产量，这与在96 h，211和174 mg / g预处理Sds的碱预处理Sds不同，分别。这些结果表明，鸡尾酒中纤维素酶的比例及其协同作用取决于用于生物质的预处理类型。因此，这种针对预处理的内部鸡尾酒设计可以大规模改善生物质的糖化作用。