An environmental friendly extraction procedure has been tested to extract phenolic compounds from H. sabdariffa calyces using pressurized GRAS solvents. A central composite rotatable design (CCRD) was performed to evaluate the influence of the main operational conditions: temperature (40–200 °C) and solvent composition based on aqueous hidroalcoholic solutions (0–100% ethanol). Phenolic composition of experimental extracts analyzed by HPLC-ESI-QTOF-MS showed that higher temperatures and greater ethanol percentages drove to solvents with lower dielectric constants, which resulted in extracts with major quantities of phenolic compounds. An exception was the extraction of cyanidin-3-sambubioside that could only be quantified in extracts performed at the lowest temperature (40 °C) due to its thermal sensibility. In addition, a RSM was carried out with the aim to maximize the extraction of total phenolic content. To this end, the predicted optimal extraction conditions by RSM were 200 °C and 100% (v/v) of ethanol. Results showed that temperature and ethanol percentage had a significant influence on the extraction of total phenolic compounds (p value < 0.05). The mathematical model pointed out 200 °C of temperature and 100% of ethanol as the optimum conditions to perform the isolation of phenolic compounds by means of pressurized GRAS solvents.

已经测试了一种环境友好的提取程序，可从s。abdariffa中提取酚类化合物使用加压的GRAS溶剂清洗花萼。进行了中央复合材料可旋转设计（CCRD），以评估主要操作条件的影响：温度（40–200°C）和基于水合醇水溶液（0–100％乙醇）的溶剂组成。通过HPLC-ESI-QTOF-MS分析的实验提取物的酚类成分表明，较高的温度和较高的乙醇含量驱使介电常数较低的溶剂进入提取物中，导致提取物中的酚类化合物含量较高。唯一的例外是花青素3-桑树生物素的提取，由于其热敏性，只能在最低温度（40°C）下进行提取。此外，进行RSM的目的是最大程度地提取总酚含量。为此，RSM预测的最佳提取条件为200°C和100％（v / v）的乙醇。结果表明，温度和乙醇百分比对总酚类化合物的提取有显着影响（p值<0.05）。数学模型指出，200℃的温度和100％的乙醇是通过加压GRAS溶剂分离酚类化合物的最佳条件。