Triazole herbicide, Thiencarbazone-methyl (TCM) applied on different crops for weedicidal activity is associated with an inherent toxicity towards bladder and urinary functionality. TCM has been first time explored for its biodegradative behavior utilizing microbes, previously isolated from soils. Simulated bio-transformation assemblies of five fungal strains; Aspergillus flavus (AF), Penicillium chrysogenum (PC), Aspergillus niger (AN), Aspergillus terrus (AT), Aspergillus fumigatus (AFu) and two bacterial strains: Xanthomonas citri (XC), Pseudomonassyringae (PS), were utilized. 10 mg/L TCM concentration was set up utilizing each microbe and analysed for 42 days. TCM bio-degradation was evaluated by UV–Visible spectrophotometery and gas chromatography mass spectroscopy. Aspergillus terrus (R² = 0.86) and Penicillium chrysogenum (R² = 0.88) exhibited highest capability to metabolize TCM while forming intermediate metabolites including; 2,4-dihydro-[1,2,4] triazol-3-one, semicarbazide and urea, methyl 4-isocyanatosulfonyl-5-methylthiophene-3-carboxylate. TCM degradation by all strains AF, AFu, AN, PC, AT, PS and XC was found to be 74, 74, 81, 95, 98, 90 and 95%, respectively after 42 days elucidating the effectiveness of all the utilized strains in degrading TCM. Current investigations can impact vital bioremediation approaches for pesticides mitigation from the ecological compartments. Furthermore, present research can be extended to the optimization of the bio-deteriorative assays to be employed on the practical scale for the successful management of environment through sustainable and cost effective ways.

三唑类除草剂噻吩甲carb甲基（TCM）应用于不同作物的除草活性与对膀胱和泌尿功能的固有毒性有关。中药是首次利用微生物（从土壤中分离出来）的生物降解特性进行研究的。模拟的五个真菌菌株的生物转化装配；黄曲霉（AF），产黄青霉（PC），黑曲霉（AN），烟曲霉（AT），烟曲霉（AFu）和两个细菌菌株：柠檬黄单胞菌（XC），假单胞菌（PS）被利用。利用每种微生物设置10 mg / L中药浓度，并分析42天。用紫外可见分光光度法和气相色谱质谱法对中药的生物降解进行了评估。曲霉（R ²  = 0.86）和产黄青霉（R ²  = 0.88）表现出最高的中药代谢能力，同时形成中间代谢产物，包括：2,4-二氢-[1,2,4]三唑-3-酮，氨基脲和脲，4-异氰酸根合磺酰基-5-甲基噻吩-3-羧酸甲酯。所有菌株AF，AFu，AN，PC，AT，PS和XC对中药的降解在42天后，发现其被发现分别为74％，74％，81％，95％，98％，90％和95％，这阐明了所利用的所有菌株在降解中药中的有效性。当前的研究可能会影响重要的生物修复方法，以缓解生态系统中的农药。此外，当前的研究可以扩展到生物降解测定的优化，以通过可持续和成本有效的方式在实际规模上成功地管理环境。