Objective

To evaluate the influence of light emitted from two Polywave®, LED light-curing units (LCU) on in vivo pulp temperature (PT) rise and signs of acute inflammatory response in pulps of human premolar having deep Class V preparations.

Methods

Sixty intact, first premolars from 15 volunteers requiring extraction received infiltrative anesthesia. A sterile thermocouple probe was inserted within the pulp tissue through a minute occlusal pulp exposure in only 45 teeth (n = 9) to continuously monitor PT (°C). A deep buccal Class V preparation was created, and the surface was exposed to light from a commercial Polywave LCU (Bluephase 20i (20i), Ivoclar Vivadent) or from an experimental LCU (Exp) using the exposure modes (EM): 1s/Exp and 2s/Exp, 10s/20i, 20s/20i, and 60s/20i. Peak PT and PT rise values above baseline (ΔT) data were evaluated using a one-way ANOVA followed by Tukey’s post-hoc test (α = 5%). Teeth used for histological and immunohistochemical analyses (n = 3) were extracted approximately 2 h after exposure to the LCU.

Results

No significant difference in peak PT and ΔT values was noted between 2s/Exp and 20s/20i groups, which both exhibited higher values than 1s/Exp and 10s/20i groups (p < 0.001). Dilated and congested blood vessels were seen after exposure to 1s/Exp, 2s/Exp, or 60s/20i EMs. The expression of IL-1β and TNF-α tended to be more intense when higher irradiance was delivered.

Significance

Although higher irradiance delivered over a short exposure caused lower PT rise than 5.5 °C, such EMs should be used with caution, as they have more potential to harm the pulp tissue.

中文翻译：

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暴露于Polywave®LED光固化单元后，具有V类制剂的前磨牙的人牙髓组织中的体内温度升高和急性炎症反应。

目的

为了评估从两个Polywave®LED光固化单元（LCU）发出的光对具有深V类制剂的人前磨牙牙髓中活体牙髓温度（PT）升高和急性炎症反应迹象的影响。

方法

来自15名需要拔牙的志愿者的60颗完整的第一前磨牙接受了浸润麻醉。通过仅在45个牙齿（n = 9）中咬合牙髓一分钟，将无菌热电偶探针插入牙髓组织内，以连续监测PT（°C）。创建了深颊颊V类制剂，并使用商业模式Polywave LCU（Bluephase 20i（20i），Ivoclar Vivadent）或实验性LCU（Exp）使用曝光模式（EM）：1s / Exp对表面进行曝光。和2s / Exp，10s / 20i，20s / 20i和60s / 20i。使用单向方差分析（ANOVA）然后进行Tukey事后检验（α），评估峰值PT和PT高于基线（ΔT）数据的上升值= 5％）。暴露于LCU大约2小时后，提取用于组织学和免疫组织化学分析的牙齿（n = 3）。

结果

在2s / Exp组和20s / 20i组之间，没有发现PT和ΔT峰值的显着差异，这两个组均显示出高于1s / Exp组和10s / 20i组的值（p <0.001）。暴露于1s / Exp，2s / Exp或60s / 20i EMs后，可见血管扩张和充血。当递送更高的辐照度时，IL-1β和TNF-α的表达趋于更强烈。

意义

尽管在短时间照射下产生较高的辐照度会导致PT升高低于5.5°C，但此类EM仍应谨慎使用，因为它们更有可能损害牙髓组织。