Novel pit and fissure sealant containing nano-CaF2 and dimethylaminohexadecyl methacrylate with double benefits of fluoride release and antibacterial function.,Dental Materials

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Novel pit and fissure sealant containing nano-CaF2 and dimethylaminohexadecyl methacrylate with double benefits of fluoride release and antibacterial function.
Dental Materials ( IF 4.6 ) Pub Date : 2020-06-19 , DOI: 10.1016/j.dental.2020.05.010
Xiuzhi Fei ₁ , Yuncong Li ₂ , Michael D Weir ₃ , Bashayer H Baras ₄ , Haohao Wang ₅ , Suping Wang ₅ , Jirun Sun ₆ , Mary A S Melo ₃ , Jianping Ruan ₇ , Hockin H K Xu ₈

Affiliation

Clinical Research Center of Shaanxi Province for Dental and Maxillofacial Diseases, Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, and Department of Preventive Dentistry, College of Stomatology, Xi'an Jiaotong University, Xi'an, Shaanxi 710004, China; Department of Advanced Oral Sciences & Therapeutics, University of Maryland School of Dentistry, Baltimore, MD 21201, USA.
Department of Prosthodontics, College of Stomatology, Xi'an Jiaotong University, Xi'an, Shaanxi 710004, China.
Department of Advanced Oral Sciences & Therapeutics, University of Maryland School of Dentistry, Baltimore, MD 21201, USA.
Department of Advanced Oral Sciences & Therapeutics, University of Maryland School of Dentistry, Baltimore, MD 21201, USA; Department of Restorative Dental Science, College of Dentistry, King Saud University, Riyadh, Saudi Arabia.
Department of Advanced Oral Sciences & Therapeutics, University of Maryland School of Dentistry, Baltimore, MD 21201, USA; State Key Laboratary of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
Volpe Research Center, American Dental Association Foundation, Frederick, MD 21704, USA.
Clinical Research Center of Shaanxi Province for Dental and Maxillofacial Diseases, Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, and Department of Preventive Dentistry, College of Stomatology, Xi'an Jiaotong University, Xi'an, Shaanxi 710004, China.
Department of Advanced Oral Sciences & Therapeutics, University of Maryland School of Dentistry, Baltimore, MD 21201, USA; Center for Stem Cell Biology & Regenerative Medicine, University of Maryland School of Medicine, Baltimore, MD 21201, USA; University of Maryland Marlene and Stewart Greenebaum Cancer Center, University of Maryland School of Medicine, Baltimore, MD 21201, USA.

Objective

Pit and fissure sealants with antibacterial and remineralization properties have broad application prospects in caries prevention. The objectives of this study were to: (1) develop a novel pit and fissure sealant containing CaF₂ nanoparticles (nCaF₂) and dimethylaminohexadecyl methacrylate (DMAHDM); and (2) investigate the effects of nCaF₂ and DMAHDM on biofilm response and fluoride (F) ion release for the first time.

Methods

Helioseal F was used as a control. Bioactive sealants were formulated with DMAHDM and nCaF₂. Flow properties, enamel shear bond strength, hardness and F ion releases were measured. Streptococcus mutans (S. mutans) biofilms were grown on sealants. Biofilm metabolic activity, lactic acid production, colony-forming units (CFU), and pH of biofilm culture medium were measured.

Results

Adding 5% DMAHDM and 20% nCaF₂ did not reduce the paste flow and enamel bond strength, compared to control (p < 0.05). Hardness of sealants with 20% nCaF₂ and DMAHDM was higher than control (p < 0.05). The F ion release from 20% nCaF₂ was much higher than that of commercial control (p < 0.05). The sealant with DMAHDM reduced the S. mutans biofilm CFU by 4 logs. The pH in biofilm medium of the new bioactive sealant was much higher (pH 6.8) than that of commercial sealant (pH 4.66) (p < 0.05).

Significance

The new bioactive pit and fissure sealant with nCaF₂ and DMAHDM achieved high fluoride release and strong antibacterial performance. This novel fluoride-releasing and antibacterial sealant is promising to inhibit caries and promote the remineralizaton of enamel and dentin.

中文翻译：

包含纳米CaF2和甲基丙烯酸二甲基氨基十六烷基酯的新型凹坑和缝隙密封剂，具有氟化物释放和抗菌功能的双重优势。

目的

具有抗菌和矿化特性的坑缝密封剂在龋齿预防中具有广阔的应用前景。这项研究的目的是：（1）开发一种新型的含有CaF ₂纳米颗粒（nCaF ₂）和甲基丙烯酸二甲基氨基十六烷基酯（DMAHDM）的裂隙密封剂。（2）首次研究nCaF ₂和DMAHDM对生物膜响应和氟离子（F）释放的影响。

方法

Helioseal F用作对照。用DMAHDM和nCaF ₂配制生物活性密封剂。测量了流动性能，搪瓷剪切粘合强度，硬度和F离子释放。变形链球菌（S. mutans）生物膜在密封胶上生长。测量生物膜代谢活性，乳酸产生，菌落形成单位（CFU）和生物膜培养基的pH。

结果

与对照相比，添加5％DMAHDM和20％nCaF ₂不会降低浆料流动性和搪瓷粘合强度（p <0.05）。含20％nCaF ₂和DMAHDM的密封胶的硬度高于对照组（p <0.05）。从20％nCaF _2中释放出的F离子远高于商业对照（p <0.05）。使用DMAHDM的密封剂使变形链球菌生物膜CFU降低了4个对数。新型生物活性密封剂在生物膜介质中的pH值（pH 6.8）远远高于商业密封剂（pH 4.66）（p <0.05）。