In this study, we prepared layer-by-layer (LbL) films from erythrosine (ERY) dye alternated with bovine serum albumin (BSA). BSA was chosen because is a model protein, and due to ability to bind with organic and inorganic molecules and transport various substances in the blood. ERY is typically used in foodstuffs, cosmetics, textiles, and medicine. It has been associated with health problems such as hyperactivity, asthma, and thyroid cancer. Dyes and other bioactive molecules bind to albumin; consequently, they contribute significantly to dye–protein interactions. The adsorption of erythrosine was monitored by UV–Vis spectrophotometry. Adsorption kinetics was fitted with the Johnson–Mehl–Avrami equation and the surface morphology of the films was investigated by atomic force microscopy. The concentration, pH, and temperature of solutions, analyzed using scaling laws, are key factors affecting the morphology of the films. The morphology of the films depends on the preparation procedures, which changes the wettability of the films. The successful preparation of LbL films from BSA and ERY demonstrates the suitability of this technique for building films of biomolecules and dyes and its strong dependence on experimental factors.

在这项研究中，我们从赤藓红（ERY）染料与牛血清白蛋白（BSA）交替制备了一层层（LbL）膜。选择BSA是因为它是一种模型蛋白，并且具有与有机和无机分子结合并在血液中转运各种物质的能力。ERY通常用于食品，化妆品，纺织品和药品。它与诸如多动症，哮喘和甲状腺癌等健康问题有关。染料和其他生物活性分子与白蛋白结合；因此，它们显着促进了染料与蛋白质的相互作用。紫外-可见分光光度法监测了赤藓红的吸附。吸附动力学符合Johnson-Mehl-Avrami方程，并通过原子力显微镜研究了膜的表面形态。溶液的浓度，pH和温度，使用缩放定律进行分析，是影响薄膜形态的关键因素。薄膜的形态取决于制备过程，这改变了薄膜的润湿性。由BSA和ERY成功制备LbL膜证明了该技术适用于构建生物分子和染料膜，并且强烈依赖于实验因素。