Irreversible electroporation (IRE) is a new technique in which a series of short pulses with high frequency electrical energy is applied on the targeted regions of cells or vesicles for their destruction or rupture formation. IRE induces lateral tension in the membranes of vesicles. We have investigated the electrostatic interaction effects on the constant electrical tension-induced rate constant of irreversible pore formation in the membranes of giant unilamellar vesicles (GUVs). The electrostatic interaction has been varied by changing the salt concentration in buffer and the surface charge density of membranes. The membranes of GUVs are synthesized by a mixture of negatively charged lipid dioleoylphosphatidylglycerol (DOPG) and neutral lipid dioleoylphosphatidylcholine (DOPC) using the natural swelling method. The rate constant of pore formation increases with the decrease of salt concentration in buffer along with the increase of surface charge density of membranes. The tension dependent probability of pore formation and the rate constant of pore formation are fitted to the theoretical equation, and obtained the line tension of membranes. The decrease in energy barrier of a prepore due to electrostatic interaction is the key factor causing an increase of rate constant of pore formation.

不可逆电穿孔（IRE）是一种新技术，其中将一系列具有高频电能的短脉冲施加到细胞或囊泡的目标区域，以破坏或破裂形成。IRE在囊泡膜上引起侧向张力。我们已经研究了静电相互作用对巨大单层囊泡（GUVs）膜中不可逆孔形成的恒定电张力诱导的速率常数的影响。通过改变缓冲液中的盐浓度和膜的表面电荷密度，可以改变静电相互作用。使用自然溶胀法，通过带负电荷的脂质二油酰基磷脂酰甘油（DOPG）和中性脂质二油酰基磷脂酰胆碱（DOPC）的混合物合成GUV的膜。孔形成速率常数随着缓冲液中盐浓度的降低以及膜表面电荷密度的增加而增加。将取决于张力的成孔概率和成孔速率常数与理论方程拟合，得到膜的线张力。由于静电相互作用而使前孔的能垒降低是导致孔形成速率常数增加的关键因素。