Recently, plenty of nanomaterials have demonstrated great promise for photothermal therapy of osteosarcoma. However, bioactive nanomaterials are still rare to function as nano-photothermal agents to thoroughly kill residual tumor cells and repair the bone defects caused by surgical removal of bone tumors. Herein, for the first time, two-dimensional (2D) borocarbonitrides (BCN) nanosheets were applied in the biomedicine to play multiple roles of photothermal agent for osteosarcoma therapy and promoter for bone regeneration. In this work, akermanite (Ca₂MgSi₂O₇, AKT) bioceramic scaffolds with pore structure of ~320 μm were first fabricated by 3D printing technology. And then a novel bifunctional platform which was marked as BCN@AKT scaffolds, was further constructed by introducing BCN nanosheets as a functional coating on AKT scaffolds, which might be attributed to the interaction between BCN with negative potential with Ca ions sites in AKT scaffolds, and the hydrogen bonding between BCN and AKT scaffolds. Notably, the unique photothermal performance of BCN@AKT scaffolds for osteosarcoma therapy was achieved due to the strong light absorption property of BCN nanosheets. In addition, the abundant hydroxyl functional group (–OH) and boron (B) element on BCN nanosheets contributed to the promotion of bone regeneration, which confirmed by in situ mineralization ability, up-regulation of fibronectin (FN) protein and activation of BMP2 signaling pathway. Therefore, the BCN engineered 3D scaffolds suggested a great integrated strategy in tumor therapy and bone regeneration.

最近，大量的纳米材料已经显示出对骨肉瘤光热疗法的巨大希望。然而，生物活性纳米材料仍很少用作纳米光热剂，以彻底杀死残留的肿瘤细胞并修复由于外科手术切除骨肿瘤而引起的骨缺损。在此，二维（2D）硼碳氮化物（BCN）纳米片首次应用于生物医学中，以发挥光热剂在骨肉瘤治疗中的作用以及骨再生的促进剂的作用。在这项工作中，钙钛矿（Ca ₂ MgSi ₂ O ₇，AKT）具有约320μm孔结构的生物陶瓷支架是首先通过3D打印技术制造的。然后，通过将BCN纳米片作为功能涂层引入AKT支架上，进一步构建一个标记为BCN @ AKT支架的新型双功能平台，这可能归因于具有负电势的BCN与AKT支架中Ca离子位点之间的相互作用，以及BCN和AKT支架之间的氢键。值得注意的是，由于BCN纳米片具有很强的光吸收特性，因此实现了BCN @ AKT支架在骨肉瘤治疗中的独特光热性能。此外，BCN纳米片上丰富的羟基官能团（–OH）和硼（B）元素有助于促进骨再生，这在原位得到证实矿化能力，纤连蛋白（FN）蛋白的上调和BMP2信号通路的激活。因此，由BCN设计的3D支架在肿瘤治疗和骨骼再生方面提出了一个很好的综合策略。