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Boosting electrochemical immunosensing performance by employing acetaminophen as a peroxidase substrate.
Biosensors and Bioelectronics ( IF 10.7 ) Pub Date : 2020-06-20 , DOI: 10.1016/j.bios.2020.112337
Kai Yan 1 , Al-Monsur Jiaul Haque 1 , Ponnusamy Nandhakumar 1 , Aman Bhatia 1 , Nam-Sihk Lee 2 , Young Ho Yoon 2 , Haesik Yang 1
Affiliation  

In horseradish peroxidase (HRP)-based electrochemical immunosensing, an appropriate HRP substrate needs to be chosen to obtain a high electrochemical signal-to-background ratio. This is limited by the unwanted electrochemical reduction of H2O2, oxidation of the substrate, and the slow electrochemical reduction of the product. Herein, we report acetaminophen (AMP) as a new HRP substrate that allows for highly sensitive immunosensing. Electrochemical behavior and immunosensing performance using AMP are compared with those using the most popular HRP substrate, hydroquinone (HQ). To maintain a high electrocatalytic activity even at an electrode modified with an immunosensing layer, an indium tin oxide electrode partially modified with reduced graphene oxide is employed. AMP allows for a higher signal-to-background ratio than HQ, because the formal potential of AMP is 0.28 V higher than that of HQ and the redox reaction of AMP is as reversible as that of HQ, resulting in a lower detection limit in a sandwich-type immunoassay using AMP for thyroid-stimulating hormone detection. The calculated detection limit is ~0.2 pg/mL. The use of AMP as an HRP substrate is especially promising for highly sensitive electrochemical immunoassays.



中文翻译:

通过使用对乙酰氨基酚作为过氧化物酶底物来提高电化学免疫传感性能。

在基于辣根过氧化物酶(HRP)的电化学免疫传感中,需要选择合适的HRP底物以获得较高的电化学信噪比。这受到H 2 O 2的不希望的电化学还原的限制,底物的氧化和缓慢的电化学还原产物。在本文中,我们报道了对乙酰氨基酚(AMP)作为一种新的HRP底物,可实现高度灵敏的免疫传感。将使用AMP的电化学行为和免疫感应性能与使用最流行的HRP底物对苯二酚(HQ)进行比较。为了即使在用免疫传感层改性的电极上也保持高的电催化活性,采用了用还原的氧化石墨烯部分改性的铟锡氧化物电极。由于AMP的形式电位比HQ高0.28 V,并且AMP的氧化还原反应与HQ一样可逆,因此AMP的信噪比比HQ高。使用AMP的夹心型免疫分析法检测甲状腺刺激激素。计算出的检出限为〜0.2 pg / mL。AMP作为HRP底物的用途对于高度灵敏的电化学免疫分析特别有希望。

更新日期:2020-06-30
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