Abstract Glucose, xylose, and fructose from biomass are renewable sources for glycerol production. Here, a TPI1 gene encoding triose phosphate isomerase (TPI) was identified in Kluyveromyces marxianus NBRC1777. The TPI1 gene was deleted in the KU70 defective K. marxianus, resulting in YZB115. YZB115 could grow with glucose, fructose, and xylose as sole carbon sources, which is contrary to the Saccharomyces cerevisiae TPI1 deletion strain. Under aerobic conditions, YZB115 fermented 80 g/L glucose, fructose, and xylose, producing 40.32, 41.84, and 18.64 g/L glycerol with productivities of 0.84, 0.50, and 0.22 g/L/h at 42 °C without by-product, respectively. The yields were about 0.5 g/g when hexoses were used, which is 98 % of the theoretical maximum. The yield decreased to 0.22 g/g when xylose was used. In summary, our study showed the potential of K. marxianus to produce glycerol and glycerol derivatives from cellulose, lignocellulosic, and inulin biomass at high temperature.

中文翻译：

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以葡萄糖、果糖和木糖为原料，通过 TPI1 缺陷型马克斯克鲁维酵母在高温下生产甘油

摘要 来自生物质的葡萄糖、木糖和果糖是甘油生产的可再生资源。在这里，在马克斯克鲁维酵母 NBRC1777 中鉴定了编码丙糖磷酸异构酶 (TPI) 的 TPI1 基因。TPI1 基因在 KU70 缺陷的马氏 K. marxianus 中被删除，导致 YZB115。YZB115 可以以葡萄糖、果糖和木糖为唯一碳源生长，这与酿酒酵母 TPI1 缺失菌株相反。在有氧条件下，YZB115 在 42 °C 下发酵 80 g/L 葡萄糖、果糖和木糖，产生 40.32、41.84 和 18.64 g/L 甘油，生产率分别为 0.84、0.50 和 0.22 g/L/h ， 分别。当使用己糖时，产率约为 0.5 g/g，为理论最大值的 98%。当使用木糖时，产率降低至 0.22 g/g。总之，