Surface modification of microfluidic chips used for making organ-on-a-chip (OOC) applications is often a time-consuming process, involving chip cleaning, ultraviolet (UV)-exposure, and steam sterilization. This work reports developing a simple, rapid, and cost-effective method that can achieve photo-activated polymerization and patterning of catecholamine materials on microfluidic chips in a single step using the UV light present in a standard biosafety cabinet. Polydimethylsiloxane (PDMS) microfluidic devices were filled with monomers of dopamine and norepinephrine, followed by exposure to UV light triggers polymerization of the material, which creates a highly viable surface for OOC applications. We examined the performance of these UV-triggered surface coatings for creating three different kinds of OOCs, where microfluidic chips were bonded and modified in three different ways: i) conventional oxygen plasma bonded microfluidic chips filled with monomer solutions and then exposed to UV to modify the surface (Plasma bonded, polymer-coated); ii) both the fluidic layer and glass substrate were exposed to UV to coat the functional layer and simultaneously allow adhesive proteins to bind the two pieces together (UV-bonded, polymer-coated); and iii) project the UV light through a mask to create fluid wall microfluidic channels on a polydimethylsiloxane (PDMS) substrate (projection coating). Cath.a.differentiated (CAD) cells seeded on UV-exposed polymer-coated surface in the three techniques showed significantly high cell viability, cell adhesion, proliferation, genetic expression, and they retained the functionality compared to uncoated PDMS. The UV-triggered surface modification technique uses a minimalist approach by using less equipment and existing infrastructure, such as a biosafety cabinet, for creating a functional OOC. This novel, simple, low-cost approach to reproducibly generating an organ-on-a-chip will facilitate the wider adoption of this technique.

的用于制造器官的微流体芯片的表面改性-上-一个-芯片（OOC）应用程序通常是一个耗时的过程，涉及芯片清洗，紫外线（UV） -曝光，并蒸汽灭菌。这项工作报告开发一个简单，快速，成本-有效的方法，可以实现照片-使用标准生物安全柜中的紫外线，一步即可激活儿茶酚胺材料在微流控芯片上的聚合和图案化。聚二甲基硅氧烷（PDMS）微流体装置中充满了多巴胺和去甲肾上腺素的单体，然后暴露于紫外线下触发了材料的聚合反应，从而为OOC应用创造了高度可行的表面。我们研究了这些UV的性能-触发的表面涂层用于创建三种不同类型的光正交码，其中微流控芯片贴合和修饰的三种不同的方式构成：i）常规的氧等离子体键合填充有单体溶液的微流体芯片，然后暴露于UV修改表面（等离子粘结，聚合物-涂层）; ii）兼具流体层和玻璃基板暴露于UV涂覆所述功能层，并且同时允许粘合剂蛋白质的两片结合在一起（UV -聚合物键合，-涂层）; iii）通过掩模投射紫外线，以在聚二甲基硅氧烷（PDMS）基材（投射涂层）上形成流体壁微流体通道。三种技术中，在Cat.a.分化（CAD）细胞上接种有UV暴露的聚合物涂层的表面均显示出很高的细胞活力，细胞粘附，增殖，遗传表达，并且与未涂层的PDMS相比，它们保留了功能。紫外线触发的表面改性技术使用了极简主义的方法，即使用更少的设备和现有的基础设施（例如生物安全柜）来创建功能性OOC。这种新颖，简单，低成本的方法可重复生成芯片上的器官，将促进该技术的广泛采用。