Abstract — The extracellular part of the ErbB2 receptor (ecdErbB2), an oncological marker and a target for therapeutic antibodies, has been expressed in eukaryotic CHO cells. The extracellular part of the ErbB2 receptor has been isolated from a culture medium by metal affinity chromatography and characterized by biochemical and immunochemical methods. It has been shown that the receptor is produced in monomeric, dimeric, and trimeric forms. The oligomeric forms have been separated by gel filtration, and their ability to bind to Herceptin, one of the main drugs in the treatment of breast cancer, has been characterized by ELISA. The receptor produced can be used for in vitro immunochemical experiments.

中文翻译：

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用于免疫生物学研究的 ErbB2 受体细胞外部分的生产

摘要 — ErbB2 受体 (ecdErbB2) 的细胞外部分是一种肿瘤标志物和治疗性抗体的靶标，已在真核 CHO 细胞中表达。ErbB2 受体的细胞外部分已通过金属亲和层析从培养基中分离出来，并通过生化和免疫化学方法进行表征。已经表明受体以单体、二聚体和三聚体形式产生。寡聚形式已通过凝胶过滤分离，并且它们与赫赛汀（治疗乳腺癌的主要药物之一）结合的能力已通过 ELISA 表征。产生的受体可用于体外免疫化学实验。