The molecular prevalence and genotypes of Giardia duodenalis in cattle were investigated. A total of 450 fecal samples were collected from cattle in three provinces of Central Anatolia from August 2017 to July 2019. Genomic DNA was extracted from the fecal samples and used in molecular analysis carried out by nested PCR analyses of the β-giardin (bg) gene of G. duodenalis. Positive samples were further analyzed by nested PCR at two gene loci (triosephosphate isomerase (tpi) and glutamate dehydrogenase (gdh)) for genotyping of G. duodenalis isolates. PCR analyses of the bg gene indicated that the overall prevalence of G. duodenalis was 30.2%. However, lower rates were determined with PCR analyses for gdh and tpi loci. The sequence analyses of the bg, gdh, and tpi genes revealed the presence of zoonotic assemblage A and livestock-specific assemblage E. Combined-sequence analyses revealed that assemblage E was the most common in the study area. Our study provides the first data on the wide prevalence of livestock-specific assemblages E in cattle in Turkey. The prevalence of assemblage A in cattle also reveals the importance of cattle for zoonotic transmission of giardiasis in Turkey.

研究了牛鞭毛贾第鞭毛虫的分子流行率和基因型。2017年8月至2019年7月，从安纳托利亚中部三个省的牛共采集了450份粪便样品。从粪便样品中提取基因组DNA，并将其用于通过巢式PCR分析β-giardin（bg）进行的分子分析G. duodenalis的基因。阳性样品通过巢式PCR在两个基因位点（磷酸三糖异构酶（tpi）和谷氨酸脱氢酶（gdh））上进一步分析，以鉴定十二指肠球菌的基因型。bg的PCR分析该基因表明十二指肠球菌的总患病率为30.2％。然而，通过PCR分析对gdh和tpi基因座确定了较低的比率。对bg，gdh和tpi基因的序列分析揭示了人畜共患病毒组合A和牲畜特定组合E的存在。组合序列分析显示组合E在研究区域中最常见。我们的研究提供了有关土耳其牲畜特定组合E广泛流行的第一批数据。牛群A的流行还揭示了牛对土耳其贾第鞭毛虫的人畜共患病传播的重要性。