Parthenolide (PTL) is a natural product from the shoots of Tanacetum parthenium, which has immunomodulatory effects in multiply type of diseases. This study aimed to explore the effect and the underlying mechanism of PTL on the anti-apoptotic and anti- inflammatory ability of tweak-induced podocytes. Conditionally immortalized mouse podocytes were incubated with Tumor necrosis factor-like weak inducer of apoptosis (Tweak, 100 ng/ml), PTL(10 µM) or Tweak + PTL for 12 h, 24 and 48 h, respectively. Podocytes viability was detected by CCK-8 assay. Tweak and Cxcl16 expression were evaluated by western blot and immunofluorescence assay. Dil-oxLDL stain was detected by immunofluorescence analysis. Intracellular Total Cholesterol (TC) content was measured through TC detection Kit. These results demonstrated that the podocytes cells viability was gradually decreased after treatment with different concentrations of Tweak (0, 50, 100, 150). Tweak and Cxcl16 protein expression in mouse podocytes treated with tweak were remarkably elevated and were found to have higher intracellular lipid accumulation compared with the control group, whereas co-administration with PTL, tweak and Cxcl16 expression as well as the intracellular lipid accumulation were notablely decreased in tweak-induced podocytes. Therefore, our conclusion was that tweak and Cxcl16 were involved in the regulation of tweak-induced podocytes injury. Meanwhile, the anti-apoptotic and anti-inflammatory effect of PTL may be correlated with the tweak and Cxcl16 expression decreased.

爬山虎内酯（PTL）是从艾叶艾草的芽中提取的天然产物，在多种疾病中具有免疫调节作用。这项研究旨在探讨PTL对调节性足细胞抗凋亡和抗炎能力的影响及其潜在机制。将条件永生的小鼠足细胞与肿瘤坏死因子样的凋亡弱诱导剂（Tweak，100 ng / ml），PTL（10 µM）或Tweak + PTL孵育12 h，24和48 h。通过CCK-8测定法检测足细胞的生存力。通过蛋白质印迹和免疫荧光测定法评估Tweak和Cxcl16的表达。通过免疫荧光分析检测Dil-oxLDL染色。通过TC检测试剂盒测量细胞内总胆固醇（TC）含量。这些结果表明，用不同浓度的Tweak（0、50、100，150）。与对照组相比，经tweak处理的小鼠足细胞中的tweak和Cxcl16蛋白表达显着升高，并且具有更高的细胞内脂质蓄积，而与PTL，tweak和Cxcl16表达的共同给药以及细胞内脂质蓄积显着降低。在微调诱导的足细胞中。因此，我们的结论是调节和Cxcl16参与调节诱导的足细胞损伤的调控。同时，PTL的抗凋亡和抗炎作用可能与调节和Cxcl16表达降低有关。调节诱导的足细胞中的调节和Cxcl16表达以及细胞内脂质积累显着降低。因此，我们的结论是调节和Cxcl16参与调节诱导的足细胞损伤的调控。同时，PTL的抗凋亡和抗炎作用可能与调节和Cxcl16表达降低有关。调节诱导的足细胞中的调节和Cxcl16表达以及细胞内脂质积累显着降低。因此，我们的结论是调节和Cxcl16参与调节诱导的足细胞损伤的调控。同时，PTL的抗凋亡和抗炎作用可能与调节和Cxcl16表达降低有关。