Emamectin, a neurotoxic agent, is a semi-synthetic insecticide that belongs to the Avermectin family and is used against helmintic infections in the Salmonidae family. Its secondary effects are not clear; thus, the aim of this study was to investigate the only effects of emamectin benzoate on various biochemical parameters (AST, ALT, GGT, total protein, albumin and glucose) in serum and expressional changes of IL-1β, TNF-α, HSP70 and IL-8 in liver and spleen. For the purpose stated above, rainbow trout (n = 15) were administered 50 μg EB per kg fish daily for 7, 14 and 21 days. The results indicated that weight gains did not change (p > 0.05), AST increased at day 21 (p < 0.05), while the changes of other biochemical parameters were not significant (p > 0.05). The changes in expression of IL-1β, TNF-α and HSP70 were significant (p < 0.05), while the changes of IL-8 expressions were not significant (p ˃ 0.05). In a conclusion, EB changed immun and stress-related gene expression in liver and spleen, and furthermore, AST changed in a dose- and time-dependent manner. The results imply that emamectin benzoate cause stress. This study is helpful to understand the effects of avermectin pharmaceutical family.

Emamectin是一种神经毒性剂，是一种半合成杀虫剂，属于Avermectin家族，用于对抗Salmonidae家族的蠕虫感染。其次要作用尚不清楚；因此，本研究的目的是研究苯甲依巴菌素对血清中各种生化参数（AST，ALT，GGT，总蛋白，白蛋白和葡萄糖）以及IL-1β，TNF-α，HSP70和肝和脾中的IL-8。为了上述目的，每天向虹鳟鱼（n = 15）施用每公斤鱼50μgEB，持续7、14和21天。结果表明，体重增加没有变化（p> 0.05），AST在第21天有所增加（p <0.05），而其他生化参数的变化不明显（p> 0.05）。IL-1β，TNF-α和HSP70的表达变化显着（p <0。05），而IL-8表达的变化不显着（p˃0.05）。总之，EB改变了肝脏和脾脏中免疫和应激相关基因的表达，此外，AST呈剂量和时间依赖性变化。该结果表明，依那莫汀苯甲酸酯引起应力。这项研究有助于了解阿维菌素药物家族的作用。