The oxidative modification of low-density lipoprotein (LDL) in the arterial wall plays a pivotal role in the initiation and progression of atherosclerosis which is a complex and progressive disorder. Paraoxonase1 (PON1), which is required for lipid metabolism, is believed to protect LDL from oxidation. The relationship between PON1 gene Leusin55Methionin (L55M) and Glutamine192Arginine (Q192R) polymorphisms in western Iranians with atherosclerosis and its association with enzyme activity and oxidized low-density lipoprotein (oxLDL) were examined in the present study. In this study, blood specimens were collected from 145 healthy individuals and 154 patients with atherosclerosis proven by angiography referred to Shahid Madani Hospital, Khorramabad, Iran. Genomic deoxy ribonucleic acid (DNA) was extracted from whole blood. For all the subjects, restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) was carried out for the detection of L55M and Q192R polymorphisms. PON1 enzyme activity and the level of oxLDL were also evaluated. There was a 3.114-fold increase in the risk of developing atherosclerosis in the subjects presenting the PON1L55M, MM genotype compared to those with the LL genotype (OR 3.114; 95% CI 1.412–6.870). PON1Q192R polymorphism in the PON1 gene was not associated with atherosclerosis. Patients with atherosclerosis had significantly higher oxLDL and reduced PON1 enzyme activity (P < 0.05) compared to the controls. There was no association between the type of genotype, enzyme activity, and oxLDL level. It has been concluded that PON1L55M polymorphism and MM genotype are associated with an increased risk of coronary artery disease (CAD) in Iranian patients with atherosclerosis. We did not find any relationship between PON1Q192R polymorphism and atherosclerosis.

动脉壁中低密度脂蛋白（LDL）的氧化修饰在动脉粥样硬化的发作和进展中起着关键作用，这是一种复杂的进行性疾病。脂质代谢所需的对氧磷酶1（PON1）被认为可以保护LDL免受氧化。在本研究中，研究了伊朗西部患有动脉粥样硬化的PON1基因Leusin55Methionin（L55M）和Glutamine192Arginine（Q192R）多态性之间的关系及其与酶活性和氧化型低密度脂蛋白（oxLDL）的关系。在这项研究中，从145例健康个体和154例经血管造影证实的动脉粥样硬化患者中收集了血液样本，这些患者被送往伊朗霍拉马巴德的Shahid Madani医院。从全血中提取基因组脱氧核糖核酸（DNA）。对于所有主题，进行了限制性片段长度多态性-聚合酶链反应（RFLP-PCR）检测L55M和Q192R多态性。还评估了PON1酶的活性和oxLDL的水平。与LL基因型的受试者相比，PON1L55M，MM基因型的受试者发生动脉粥样硬化的风险增加了3.114倍（OR 3.114； 95％CI 1.412–6.870）。PON1基因中的PON1Q192R多态性与动脉粥样硬化无关。与对照组相比，患有动脉粥样硬化的患者的oxLDL明显升高，PON1酶活性降低（P <0.05）。基因型类型，酶活性和oxLDL水平之间没有关联。已经得出结论，在伊朗的动脉粥样硬化患者中，PON1L55M多态性和MM基因型与冠状动脉疾病（CAD）的风险增加相关。我们未发现PON1Q192R多态性与动脉粥样硬化之间有任何关系。