A silver zeolite (AgY) nanocomposite was prepared and applied for construction of a modified carbon paste electrode (AgY/CPE) for sensing tryptophan (Trp) in real samples by using differential pulse voltammetry (DPV). AgY nanocomposite was prepared with a simple method from nano-structured sodium zeolite (NaY) and characterized by X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), Energy dispersive X-ray analysis (EDX). Particle size of the prepared AgY was in the range between 17.8 and 44.7 nm. Fourier transform infrared (FTIR) and fluorescence spectra AgY and AgY/Trp nanocomposites reveals interaction between AgY and Trp. Cyclic voltammetry (CV) was used for evaluating the electrode behaviour. Effect of potential scan rate on the electrode response was also studied. The proposed electrode revealed a suitable current response at scan rate of 0.1 V.s⁻¹. A descriptive mechanism was proposed for explaining the electrode response toward Trp based on the CV experiments. Effect of pH on the response of the electrode was also studied. The modified carbon paste electrode revealed a good response to Trp at pH of 6.9 in a phosphate buffer solution (PBS), in the range of Trp concentration between 10 nM and 1.2 µM. The detection limit of the electrode was 6.3 nM and it was successfully applied for the determination of Trp in milk and samples. The accuracy and precision of the results were evaluated with statistical methods.

制备了一种银沸石（AgY）纳米复合材料，并通过使用差分脉冲伏安法（DPV）将其应用于构造用于感测真实样品中色氨酸（Trp）的碳糊电极（AgY / CPE）。AgY纳米复合材料采用纳米结构钠沸石（NaY）的简单方法制备，并通过X射线衍射（XRD），场发射扫描电子显微镜（FESEM），能量色散X射线分析（EDX）进行了表征。制备的AgY的粒度在17.8和44.7nm之间的范围内。AgY和AgY / Trp纳米复合材料的傅里叶变换红外（FTIR）和荧光光谱揭示了AgY和Trp之间的相互作用。循环伏安法（CV）用于评估电极性能。还研究了电势扫描速率对电极响应的影响。^-1。提出了一种描述机制，用于基于CV实验解释电极对Trp的响应。还研究了pH对电极响应的影响。修饰的碳糊电极在磷酸盐缓冲溶液（PBS）中的pH值为6.9时，在10nM至1.2 µM的Trp浓度范围内，显示出对Trp的良好响应。电极的检出限为6.3 nM，已成功用于牛奶和样品中Trp的测定。结果的准确性和准确性用统计方法进行了评估。