Absent in melanoma 2 (AIM2) has been reported to be an important inflammasome component that exerts tumor suppression in several tumors. However, whether CCL19/CCR7/AIM2 is involved in the progression of GC still remains unclear. Quantitative real-time and ELISA assay were used to determine the expressions of AIM2, CCL19 and CCR7 in GC tissues and cell lines. CCK-8, Edu staining, flow cytometry, Transwell assay, and tumorigenesis in nude mice were used to explore the function of AIM2 and CCL19 in vitro and in vivo. Apoptosis and inflammation-related biomarkers were detected by Western blot and ELISA assay. H&E staining was used to assess the histological changes in the subcutaneous tumor model. Immunohistochemistry (IHC) was used to evaluate the expression of Ki-67. We found that expression levels of AIM2, CCL19 and CCR7 were obviously lower in early GC tissues than those in progressive GC tissues. In vitro assays revealed that CCL19 treatment could enhance the suppressive effects of AIM2 overexpression on cell proliferation, migration, and invasion through CCR7. An in vivo assay also demonstrated that silencing of AIM2 reversed the suppressive effects of CCL19 on tumor growth. Collectively, CCL19 overexpression significantly inhibited GC cell proliferation and tumor growth in vitro and in vivo by up-regulating the CCR7/AIM2 pathway. Thus, CCL19 activated CCR7/AIM2 signaling pathway and it may be a potential therapeutic approach for GC therapy.

据报道，黑色素瘤2（AIM2）缺失是一种重要的炎症小体成分，可在多种肿瘤中发挥抑瘤作用。但是，尚不清楚CCL19 / CCR7 / AIM2是否参与GC的进展。实时定量和ELISA法测定AIM2，CCL19和CCR7在GC组织和细胞系中的表达。使用CCK-8，Edu染色，流式细胞仪，Transwell分析和裸鼠体内的肿瘤发生来探讨AIM2和CCL19在体内和体外的功能。Western blot和ELISA法检测细胞凋亡和炎症相关生物标志物。H＆E染色用于评估皮下肿瘤模型的组织学变化。免疫组织化学（IHC）用于评估Ki-67的表达。我们发现AIM2的表达水平 早期GC组织中的CCL19和CCR7明显低于进行性GC组织中的CCL19和CCR7。体外测定显示，CCL19处理可以增强AIM2过表达对细胞通过CCR7增殖，迁移和侵袭的抑制作用。体内试验还表明，沉默AIM2可逆转CCL19对肿瘤生长的抑制作用。集体地，CCL19的过表达通过上调CCR7 / AIM2途径在体内外显着抑制GC细胞增殖和肿瘤生长。因此，CCL19激活了CCR7 / AIM2信号通路，可能是GC治疗的潜在治疗方法。和通过CCR7的入侵。体内试验还表明，沉默AIM2可逆转CCL19对肿瘤生长的抑制作用。集体地，CCL19的过表达通过上调CCR7 / AIM2途径在体内外显着抑制GC细胞增殖和肿瘤生长。因此，CCL19激活了CCR7 / AIM2信号通路，可能是GC治疗的潜在治疗方法。和通过CCR7的入侵。体内试验还表明，沉默AIM2可逆转CCL19对肿瘤生长的抑制作用。集体地，CCL19的过表达通过上调CCR7 / AIM2途径在体内外显着抑制GC细胞增殖和肿瘤生长。因此，CCL19激活了CCR7 / AIM2信号通路，可能是GC治疗的潜在治疗方法。