Genetic linkage map of pear ((Pyrus pyrifolia × P. communis) × P. pyrifolia) was constructed to validate the effectiveness of the two-enzyme approach in GBS library preparation. In addition, allele inheritance was analyzed to investigate the usefulness of the pear pseudo-BC1 in genetic analysis. A total of 905 GBS-SNPs and 69 SSRs were anchored in 17 linkage groups with total genetic distance of 1760.1 cM and average marker distance of 1.88 cM. The genetic linkage map represents 80.5% of pear genome. GBS two-enzyme approach improved marker density and genome coverage compared to the single-enzyme approach. In addition, the inheritance analysis of SSR alleles demonstrated that pseudo-BC could allow genetic dissection of donor parent specific trait segregating in the F2. The high-resolution genetic linkage map of pear pseudo-BC1 will be used for candidate gene identification. Moreover, genetic analysis of donor parent specific traits segregating in the pseudo-BC1 hybrids will promote marker development for molecular breeding in pear.

中文翻译：

---

使用GBS-SNPs和SSRs构建梨假BC1((Pyrus pyrifolia × P. community) × P. pyrifolia)的高分辨率遗传连锁图谱

构建梨 ((Pyrus pyrifolia × P. community) × P. pyrifolia) 的遗传连锁图，以验证双酶方法在 GBS 文库制备中的有效性。此外，分析了等位基因遗传以研究梨假 BC1 在遗传分析中的有用性。共有 905 个 GBS-SNP 和 69 个 SSR 锚定在 17 个连锁群中，总遗传距离为 1760.1 cM，平均标记距离为 1.88 cM。遗传连锁图代表了梨基因组的 80.5%。与单酶方法相比，GBS 双酶方法提高了标记密度和基因组覆盖率。此外，SSR 等位基因的遗传分析表明，假 BC 可以允许对 F2 中分离的供体亲本特定性状进行遗传解剖。梨假BC1的高分辨率遗传连锁图将用于候选基因鉴定。此外，对在假 BC1 杂种中分离的供体亲本特定性状的遗传分析将促进梨分子育种的标记开发。