Seven lignans including four lignan glycosides and three lignans were isolated from 70% methanolic extract of defatted sesame seeds. The chemical structures of the isolated compounds were identified by UV, ¹H and ¹³C NMR, and HR-MS spectra. To overcome the difficulty and high cost for the preparation of lignans, the single reference standard method for the simultaneous determination of multi-components (SSDMC) with high-performance liquid chromatography (HPLC) was developed for simultaneous quantification of four lignin glycosides in sesame seeds. All four lignin glycosides had good linear concentration-measurement relationships (r² ≥ 0.9992). The relative deviation of the method was less than 4.9% for intra- and inter-day assays and the average recovery varied from 93.3 to 101.2%. The method was also successfully applied to determine four lignan glycosides in eighteen white sesame seeds and ten black sesame seeds. The results demonstrated that the single reference standard method could be used for quality control of sesame seeds and related products since the reference standards are not commercially available.

从脱脂芝麻的70％甲醇提取物中分离出7种木脂素，包括4种木脂素苷和3种木脂素。通过UV，¹ H和¹³ C NMR和HR-MS光谱鉴定分离出的化合物的化学结构。为了克服制备木脂素的困难和高成本，开发了一种同时测定芝麻中四种木质素苷的高效液相色谱（HPLC）同时测定多组分（SSDMC）的单一参考标准方法。 。四种木质素苷均具有良好的线性浓度-测量关系（r ²≥0.9992）。对于日内和日间分析，该方法的相对偏差小于4.9％，平均回收率从93.3％到101.2％不等。该方法还成功地用于测定十八个白芝麻和十个黑芝麻中的四种木脂素苷。结果表明，单一参考标准方法可用于芝麻和相关产品的质量控制，因为参考标准无法从市场上买到。