Photothermal effect of nanomaterial has been applied to the establishment of rapid detection method such as test strip. Previously, the detector for the photothermal test strip method mainly was a bulky thermal imaging camera or an infrared-sensing temperature sensor of which the detection data obtained is unstable due to its susceptibility to environmental changes. Herein, a resistive temperature sensor which is more stable and less susceptible to external environment was adopted. A test strip was used to detect Salmonella enterica Typhimurium (Salmonella typhimurium). The carboxylated graphene oxides (GOs), which have a more excellent photothermal effect, labeled with anti-Salmonella typhimurium antibodies, were used as a nanomarker to produce thermal signal. Subsequently, temperature variation signal (ΔT) generated by carboxylated GOs exposed to 808 nm laser was detected by conventional resistive temperature sensor directly. Under optimal conditions, a standard curve of ΔT with respect to the different numbers of Salmonella typhimurium was established, the detection limit was 10⁴ CFU·mL^-1 and the detection time was 20 min. The proposed detection method with high sensitivity, specificity, and efficiency can be used as a novel and on-site screening technology to detect Salmonella typhimurium in food, environmental, or clinical samples.

纳米材料的光热效应已被用于建立快速检测方法如试纸条。以前，用于光热测试条方法的检测器主要是大体积的热成像相机或红外感测温度传感器，由于其对环境变化的敏感性，所获得的检测数据不稳定。在此，采用了更稳定且对外部环境不敏感的电阻式温度传感器。用试纸条检测鼠伤寒沙门氏菌（鼠伤寒沙门氏菌）。用抗鼠伤寒沙门氏菌标记的羧化氧化石墨烯（GOs），具有更出色的光热效应抗体被用作产生热信号的纳米标记。随后，直接由常规电阻温度传感器检测由暴露于808 nm激光的羧化GO产生的温度变化信号（ΔT）。在最佳条件下，建立了关于鼠伤寒沙门氏菌不同数量的ΔT标准曲线，检出限为10 ⁴ CFU·mL ^-1，检出时间为20 min。所提出的具有高灵敏度，特异性和效率的检测方法可作为一种新颖的现场筛选技术来检测食品，环境或临床样品中的鼠伤寒沙门氏菌。