Fire blight disease, caused by plant pathogenic bacteria Erwinia amylovora represents a global threat for pome fruit industry, affecting yield and causing serious economic damage. During six year period (2011–2016), a total of 45 isolates typical for E. amylovora were isolated from four different host plants (apple, pear, quince and medlar) in 21 localities within four Serbian regions (Southeast, Central, West, and North). Isolates were primarily identified using biochemical and serological tests, polymerase chain reaction (PCR) with species-specific primer pair (A/B) and their pathogenicity. Polyclonal antibodies used in DAS ELISA and IF were found to be specific and sensitive enough for rapid E. amylovora detection. In virulence test performed on immature pear fruitlets, higher variability (CV) was found between regions than among hosts from which isolates originated. Slighter virulence of isolates originated from North region of Serbia (Vojvodina) was found in comparison to the isolates from Central Serbia, which were the most variable. Principal components analysis (PCA) segregated quince as a host among the most prevalent isolates in group of highly virulent, but failed to identify difference related to geographic origin of Serbian isolates. The same results enabled multivariate statistical analysis. Different DNA fingerprinting techniques such as repetitive element sequence based PCR (rep-) with BOX, ERIC, REP, GTG₅ and SERE primers, then Random Amplified Polymorphic DNA (RAPD) with M13-PCR primer revealed genetic homogeneity among all Serbian E. amylovora isolates. ERIC, SERE and M13 primers generated the most complex and visually perceptible patters. Restriction fragment length polymorphism analysis (RFLP) of gyrA and recA genes, digested with three restriction endonucleases BstUI, BgIII and BsmI and rpoS gene digested with BstUI gave the unuque, characteristic patterns for all Serbian isolates.

由植物病原性细菌欧文氏小球藻引起的火疫病代表着对石榴果业的全球威胁，影响了产量并造成了严重的经济损失。在六年期间（2011年至2016年），从四个塞尔维亚地区（东南部，中部，西部，南部和北部）的21个地区的四种不同寄主植物（苹果，梨，木瓜和枸杞）中分离出总共有45种典型的支链球菌。和北部）。分离物主要通过生化和血清学测试，带有物种特异性引物对（A / B）的聚合酶链反应（PCR）及其致病性进行鉴定。发现用于DAS ELISA和IF的多克隆抗体具有足够的特异性和灵敏性，可用于快速的支链淀粉检测。在对未成熟梨子小果进行的毒力测试中，发现区域之间的变异性（CV）比源自分离株的宿主之间的变异性更高。与变异最大的来自塞尔维亚中部的分离株相比，发现来自塞尔维亚北部地区（Vojvodina）的分离株毒性较低。主成分分析（PCA）将木瓜作为宿主，隔离在高毒力组中最流行的分离株中，但未能鉴定与塞尔维亚分离株的地理起源有关的差异。相同的结果可以进行多元统计分析。不同的DNA指纹技术，例如具有BOX，ERIC，REP，GTG _5的基于重复元素序列的PCR（rep-）和SERE引物，然后带有M13-PCR引物的随机扩增多态性DNA（RAPD）显示了所有塞尔维亚支链淀粉支原体分离株之间的遗传同源性。ERIC，SERE和M13引物产生了最复杂且在视觉上可感知的图案。用三种限制性内切酶Bst UI，BgI II和Bsm I消化的gyrA和recA基因的限制性片段长度多态性分析（RFLP），以及用Bst UI消化的rpoS基因，为所有塞尔维亚分离株提供了独特的特征性模式。