Biodiesel production from non-edible feedstocks, e.g., Jatropha curcas, by lipase-producing bacteria is considered a sustainable measure to reduce food versus fuel competition and dependency on fossil fuels. In this study, lipase-producing bacterial strains were isolated from soil contaminated with oil. Their biochemical and molecular identification was performed and their capacity to produce biodiesel from J. curcas seed oil was determined. Plackett-Burman and central composite designs were employed to optimize various factors for whole cell-based transesterification of J. curcas seed oil. Brevibacterium SB11 MH715025 and Pseudomonas SB15 MH715026 strains produced the highest volumetric yield of biodiesel (~ 97%). Pseudomonas SB15 MH715026 was identified as the most potent strain with the most optimum biodiesel yield on 37 °C, oil to methanol molar ratio of 1:9, and agitation at 100 rpm. Confirmation of fatty acid methyl esters was done through Fourier transform infrared spectroscopy. The infrared spectra were in the ranges 1735–1750 at cm⁻¹ and 1300–1000 cm⁻¹ that corresponds to C=O and C-O functional groups, respectively. The quality of biodiesel was evaluated and the fuel properties were determined for acid value, pour and cloud point, peroxide value, boiling point, and specific gravity. The values were 0.44 ± 0.1 mg potassium hydroxide (KOH)/g, 3 ± 0.1 °C, 4.1 ± 0.4 °C, 1.41 ± 0.1 milliequivalent (Meq) O₂/kg, 260 ± 1 °C, and 0.87 ± 0.02 kg/m³, respectively. The fuel properties of biodiesel produced during this study were in accordance with quality standards specified by the American Society for Testing and Materials (ASTM D6751) and European Norms (EN-14103).

由产生脂肪酶的细菌从非食用原料（例如麻疯树）生产生物柴油被认为是减少食物与燃料竞争以及对化石燃料的依赖的可持续措施。在这项研究中，从受油污染的土壤中分离出产生脂肪酶的细菌菌株。他们进行了生化和分子鉴定，并确定了他们从麻疯树种子油生产生物柴油的能力。采用Plackett-Burman和中央复合材料设计来优化麻疯树种子油基于全细胞的酯交换反应的各种因素。短杆菌SB11 MH715025和假单胞菌SB15 MH715026菌株产生的生物柴油体积产量最高（〜97％）。假单胞菌SB15 MH715026被认为是最有效的菌株，在37°C时生物柴油的收率最高，油与甲醇的摩尔比为1：9，并在100 rpm搅拌下。脂肪酸甲酯的确认通过傅里叶变换红外光谱法进行。红外光谱在cm ^-1和1300–1000 cm ^-1的范围内在1735-1750范围内分别对应于C ＝ O和CO官能团。评价了生物柴油的质量，并确定了酸值，倾点和浊点，过氧化物值，沸点和比重的燃料特性。值为0.44±0.1 mg氢氧化钾（KOH）/ g，3±0.1°C，4.1±0.4°C，1.41±0.1毫当量（Meq）O ₂ / kg，260±1°C和0.87±0.02 kg / m ³。在这项研究中生产的生物柴油的燃料特性符合美国测试和材料协会（ASTM D6751）和欧洲规范（EN-14103）规定的质量标准。