The study provides data on antibiotic resistance as well as the virulence characteristics of Aeromonas caviae isolated from raw and treated wastewater. The isolates were identified as A. caviae by 16S rRNA gene sequencing. In the analyzed strains, high frequency for the following genes was observed: aac(6′)-Ib-cr, qnrB, and qnrD. The presence of qnrA and ogxB genes was not found in any strain. The higher frequency of the investigated genes was observed in strains from raw wastewater (RW). The strains of A. caviae showed multiple antibiotic resistance evaluated by the disk diffusion method. Multiple antibiotic resistance indices ranged from 0.36 to 0.69. Susceptibility to six heavy metals (Cd⁺², Zn⁺², Cu⁺², Co⁺², Mn⁺², and Ni⁺²) was recorded for all the isolates. The order of metal resistance of A. caviae was Co > Cu > Zn > Cd > Ni > Mn. All the strains of A. caviae showed β-hemolytic activity. Enzymes of amylase, cellulase, and lipase were produced by all isolates. Only the strains from RW had the ability to form biofilms and showed motility. The obtained results indicate that wastewater is a potential source and/or reservoir of virulent and multidrug-resistant A. caviae as “high-risk isolates.”

中文翻译：

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废水鱼气单胞菌分离株对氟喹诺酮类药物的耐药性和毒力特性

该研究提供了关于抗生素耐药性的数据，以及从原废水和处理过的废水中分离出来的鱼气单胞菌的毒力特征。通过 16S rRNA 基因测序，分离物被鉴定为A. caviae。在分析的菌株中，观察到以下基因的高频率：aac(6')-Ib-cr、qnr B 和qnr D。在任何菌株中均未发现qnr A 和ogx B 基因的存在。在来自原废水 (RW) 的菌株中观察到更高频率的研究基因。A. caviae菌株显示出通过圆盘扩散法评估的多种抗生素耐药性。多项抗生素耐药性指数介于 0.36 至 0.69 之间。记录了所有分离株对六种重金属（Cd ⁺²、Zn ⁺²、Cu ⁺²、Co ⁺²、Mn ⁺²和Ni ⁺²）的敏感性。A. caviae的金属电阻顺序为Co>Cu>Zn>Cd>Ni>Mn。A. caviae 的所有菌株显示出β-溶血活性。所有分离株均产生淀粉酶、纤维素酶和脂肪酶。只有来自 RW 的菌株具有形成生物膜的能力并显示出运动性。获得的结果表明，废水是具有毒性和多重耐药性的A. caviae作为“高风险分离株”的潜在来源和/或储存库。