The synergistic effects of transition metal based nanocomposites are known to possess enhanced antibacterial activities. However, in-depth analysis of the relative antibacterial performance of some of the prominent nanocomposites remains unavailable. This study compares the antibacterial activity of two separate nanocomposites, which are copper oxide with silver (CuO/Ag) and zinc oxide with silver (ZnO/Ag). The individual CuO/Ag and ZnO/Ag nanocomposites were synthesised by a mixed wet-chemical method. The resulting particles were analysed by XRD, XRF, TEM, UV-Vis spectrophotometer, BET, and FTIR. The antibacterial activity of the nanoparticles were tested on Gram-negative and Gram-positive bacteria, Escherichia coli (ATCC25922) and Staphylococcus aureus (ATCC25923), respectively, using the Kirby–Bauer disc diffusion and the microdilution methods. The Kirby–Bauer disc diffusion test results had the same minimum inhibition concentration (MIC) value for both CuO/Ag and ZnO/Ag against E. coli and S. aureus, which was 0.25 mg/ml. The applied nanocomposites using microdilution showed that CuO/Ag had approximately 98.8% and 98.7% efficiency on the respective Gram-positive and Gram-negative bacterial species, while ZnO/Ag achieved 91.7% and 89.3% efficiency, respectively, against the Gram-positive and Gram-negative bacterial species. This study presents a novel approach for relative analysis of the performance efficiency of transition metal based nanocomposites.

中文翻译：

---

CuO / Ag和ZnO / Ag纳米复合材料抗菌活性的比较研究

已知基于过渡金属的纳米复合材料的协同作用具有增强的抗菌活性。但是，尚无法对某些著名的纳米复合材料的相对抗菌性能进行深入分析。这项研究比较了两种单独的纳米复合材料的抗菌活性，这两种复合材料分别是：氧化铜和银（CuO / Ag）和氧化锌和银（ZnO / Ag）。通过混合湿化学方法合成了单独的CuO / Ag和ZnO / Ag纳米复合材料。通过XRD，XRF，TEM，UV-Vis分光光度计，BET和FTIR分析所得颗粒。测试了纳米颗粒对革兰氏阴性和革兰氏阳性细菌，大肠杆菌（ATCC25922）和金黄色葡萄球菌的抗菌活性。（ATCC25923），分别使用Kirby-Bauer圆盘扩散法和微量稀释法。Kirby-Bauer椎间盘扩散测试结果对大肠杆菌和金黄色葡萄球菌的CuO / Ag和ZnO / Ag的最小抑菌浓度（MIC）值相同，均为0.25 mg / ml。使用微稀释法施加的纳米复合材料显示，CuO / Ag对革兰氏阳性和革兰氏阴性菌的效率分别约为98.8％和98.7％，而ZnO / Ag对革兰氏阳性菌的效率分别为91.7％和89.3％。和革兰氏阴性细菌。这项研究为过渡金属基纳米复合材料的性能效率提供了一种相对分析的新方法。