Imaging of cerebral vasculature is impeded with the existing fluorescence microscopy methods due to intense light scattering in living tissues and the need for highly invasive craniotomy procedures to resolve structures on a capillary scale. We propose a widefield fluorescence localization microscopy technique for high-resolution transcranial imaging and quantitative assessment of cortical perfusion in mice. The method is based on tracking single fluorescent microparticles sparsely distributed in the blood stream using a simple CMOS camera and a continuous-wave laser source. We demonstrate quantitative transcranial in vivo mapping of the blood flow velocity and direction at capillary level resolution (5 µm) across the entire cortex. The new technique opens a new high-resolution transcranial window into the brain function in health and disease.

中文翻译：

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广域荧光定位显微镜用于经颅成像的毛细管灌注皮质灌注。

由于活组织中强烈的光散射以及需要高侵入性颅骨切开术来解决毛细血管规模的结构，现有的荧光显微镜方法阻碍了脑血管成像。我们提出了一种宽视野荧光定位显微镜技术，用于高分辨率经颅成像和小鼠皮层灌注的定量评估。该方法基于使用简单的CMOS相机和连续波激光源跟踪稀疏分布在血流中的单个荧光微粒的方法。我们证明了体内定量经颅在整个皮层中以毛细管水平分辨率（5 µm）绘制血流速度和方向。新技术为健康和疾病的脑功能打开了一个新的高分辨率经颅窗口。