ABSTRACT As stress-inducible proteins, dehydrins exert functional protective role by alleviating the cell damage when plants are suffering from the stresses. However, the upstream regulatory mechanism of these proteins is not very clear. To unravel the regulatory mechanism of dehydrin, a screen of wheat cDNA library from cold and PEG-treated wheat seedlings was performed and a transcription factor TaERF4a (GenBank NO. AFP49822.1) interacting with wheat dehydrin wzy1-2 gene (Gene ID: 100037544) promoter was identified by yeast one-hybrid assay. The regulator TaERF4a and the wzy1-2 gene can respond to the abiotic stress, the induced transcripts of these two genes exhibit a similar expression trend under adverse environmental conditions. In planta, the dual luciferase transient assay analysis showed that TaERF4a can positively regulate the expression of WZY1-2 dehydrin. Therefore, the obtained results suggest that ERF transcription factor can regulate the expression level of the dehydrin gene in wheat.

中文翻译：

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ERF型转录因子参与小麦脱水蛋白wzy1-2基因的调控

摘要 作为胁迫诱导蛋白，脱水素通过减轻植物遭受胁迫时的细胞损伤而发挥功能性保护作用。然而，这些蛋白质的上游调控机制还不是很清楚。为了解开脱水素的调控机制，对冷和 PEG 处理的小麦幼苗的小麦 cDNA 文库进行了筛选，转录因子 TaERF4a（GenBank NO. AFP49822.1）与小麦脱水素 wzy1-2 基因（基因 ID：100037544）相互作用。 ) 启动子通过酵母单杂交试验鉴定。调控因子 TaERF4a 和 wzy1-2 基因可以响应非生物胁迫，这两个基因的诱导转录本在不利的环境条件下表现出相似的表达趋势。在植物中，双荧光素酶瞬时测定分析表明TaERF4a可以正调控WZY1-2脱水蛋白的表达。因此，所得结果表明ERF转录因子可以调节小麦中脱水素基因的表达水平。