Retene (1-methyl-7-isopropyl-phenanthrene, RET) is an alkyl polycyclic aromatic hydrocarbon (PAH) with environmental risk to aquatic animals. Takifugu obscurus is a migratory fish species with high economic and ecological value. To assess the toxic effects of RET on molecular metabolism, juvenile T. obscurus in this study were acutely exposed to 44.30 µg/L of RET for four days. The transcriptome profiles of livers were compared between RET treatment group and the control, and the results revealed that 1,897 genes were significantly differentially expressed (DEGs) after exposure to RET, which enriched 17 KEGG pathways. Among these, glycerolipid metabolism, glycerophospholipid metabolism, insulin signaling pathway, and FOXO signaling pathways were significantly activated. Further exploration indicated that RET exposure disrupted glucose metabolism, stimulated insulin metabolism, and activated cell proliferation genes. Overall, these findings help explain the molecular mechanisms underlying RET toxicity, and may offer evidence to support T. obscurus protection.

t（1-甲基-7-异丙基菲，RET）是烷基多环芳烃（PAH），对水生动物具有环境风险。aki鱼（Takifugu obscurus）是一种具有高经济和生态价值的value游鱼类。要评估RET对分子代谢的研究，T。obscurus在这项研究中，急性暴露于44.30 µg / L的RET持续四天。比较了RET治疗组和对照组的肝脏转录组谱，结果表明，暴露于RET后，有1,897个基因显着差异表达（DEG），这丰富了17条KEGG通路。其中，甘油脂代谢，甘油磷脂代谢，胰岛素信号传导途径和FOXO信号传导途径被显着激活。进一步的研究表明，RET暴露会破坏葡萄糖代谢，刺激胰岛素代谢并激活细胞增殖基因。总体而言，这些发现有助于解释RET毒性的分子机制，并可能提供证据支持隐性T. obscusus保护。