ABSTRACT

Background

Periodontopathic bacteria Porphyromonas gingivalis in humans and Porphyromonas gulae in animals are phylogenetically close and commonly have FimA and Mfa1 fimbriae. However, little is known about how fimA and mfa1 are phylogenetically different between P. gingivalis and P. gulae. Here, we examined phylogenetic diversity in their fim and mfa gene clusters.

Methods

Twenty P. gulae strains were isolated from the periodontal pocket of 20 dogs. For their genomic information, along with 64 P. gingivalis and 11 P. gulae genomes, phylogenetic relationship between the genotypes of fimA and mfa1 was examined. Variability of amino acid sequences was examined in the three-dimensional structure of FimA. The distance between strains was calculated for fim and mfa genes.

Results

Some fimA genotypes in P. gulae were close to particular types in P. gingivalis. Two types of mfa1 were classified as 70-kDa and 53-kDa protein-coding mfa1. The variable amino acid positions were primarily at the outer part of FimA. The genes encoding the structural proteins and the main component were similarly distant from the reference strain in P. gingivalis, but not in P. gulae.

Conclusions

The differences in the gene clusters between P. gingivalis and P. gulae may result in their host specificity.

中文翻译：

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牙龈卟啉单胞菌和牙龈卟啉单胞菌之间 fim 和 mfa 基因簇的系统发育多样性是宿主特异性的潜在原因。

摘要

背景

人类的牙周病细菌牙龈卟啉单胞菌和动物的牙龈卟啉单胞菌在系统发育上很接近，通常具有 FimA 和 Mfa1 菌毛。然而，关于fimA和mfa1在P. gingivalis和P. gulae之间的系统发育差异方面知之甚少。在这里，我们检查了它们的fim和mfa基因簇中的系统发育多样性。

方法

从 20 只狗的牙周袋中分离出20株P. gulae 。对于它们的基因组信息，连同 64 个牙龈卟啉单胞菌和 11个牙龈卟啉单胞菌基因组，检查了fimA和mfa1基因型之间的系统发育关系。在 FimA 的三维结构中检查了氨基酸序列的变异性。计算fim和mfa基因的菌株之间的距离。

结果

P. gulae中的一些 fimA基因型与P. gingivalis中的特定类型接近。两种类型的mfa1被分类为 70-kDa 和 53-kDa 蛋白质编码mfa1。可变氨基酸位置主要位于 FimA 的外部。编码结构蛋白和主要成分的基因在牙龈卟啉单胞菌中与参考菌株的距离相似，但在牙龈卟啉单胞菌中则不然。

结论

P. gingivalis和P. gulae之间基因簇的差异可能导致它们的宿主特异性。