Abstract

The present study investigated the hepatoprotective effects of seabuckthorn (Hippophae rhamnoides) leaf-extract (SLE) supplementation in lead acetate-intoxicated Wistar rats. Adult male Wistar rats (n = 35) were divided into five equal groups as per completely randomized design. Group I was kept as a control, group II received 250 ppm lead acetate in drinking water, and group III received SLE at 100 mg/kg body weight per os. Animals in group IV and group V received lead acetate at 250 ppm in drinking water for the first 45 days. Additionally, group IV received SLE at 100 mg/kg body weight per os throughout the experiment, whereas group V received SLE at 100 mg/kg body weight per os during the last 15 days of the trial. Blood samples were collected on day zero and at the 45th and 60th day to study the liver function enzymes. Lead exposure caused increase in aspartate aminotransferase (AST), alanine aminotransferase (ALT), acid phosphatase (ACP), and alkaline phosphatase (ALP) activity, and liver weight, and hepatic oxidative stress in lead acetate-intoxicated groups (groups II, IV, and V) as compared to group I. SLE on simultaneous supplementation with lead acetate (group IV) exerted a protective effect against lead toxicity. SLE supplemented after the establishment of lead acetate-toxicity (group V) also reduced the AST, ALT, and ALP activity and hepatic oxidative stress, indicating its ameliorative effect. SLE supplementation at 100 mg/kg body weight per os protects against hepatic damage caused by 250 ppm lead acetate in the drinking water of Wistar rats.

摘要

本研究调查了沙棘(沙棘)叶提取物 (SLE) 补充剂对醋酸铅中毒的 Wistar 大鼠的保肝作用。成年雄性 Wistar 大鼠 ( n  = 35) 按照完全随机设计分为五个相等的组。第 I 组作为对照，第 II 组接受 250 ppm 醋酸铅的饮用水，第 III 组接受 100 mg/kg 体重/口的 SLE 。IV 组和 V 组的动物在前 45 天接受饮用水中浓度为 250 ppm 的醋酸铅。此外，在整个实验过程中，IV 组接受了 100 mg/kg 体重口服的SLE，而 V 组接受了 100 mg/ kg体重口服的 SLE在审判的最后 15 天。在第 0 天和第 45 天和第 60 天采集血样以研究肝功能酶。铅暴露导致醋酸铅中毒组（第 II、IV 组）的天冬氨酸氨基转移酶 (AST)、丙氨酸氨基转移酶 (ALT)、酸性磷酸酶 (ACP) 和碱性磷酸酶 (ALP) 活性以及肝脏重量和肝脏氧化应激增加, 和 V) 与 I 组相比。SLE 同时补充醋酸铅（IV 组）对铅毒性具有保护作用。在醋酸铅中毒（组 V）后补充 SLE 也降低了 AST、ALT 和 ALP 活性和肝脏氧化应激，表明其有改善作用。口服 100 mg/ kg体重的 SLE 补充剂防止 Wistar 大鼠饮用水中 250 ppm 醋酸铅引起的肝损伤。