Abstract

Analytical methodologies to accurate quantify enantiomers are nowadays imperative in different areas such as pharmaceutical, agrochemicals, forensic toxicology, food and environmental analysis, among others. This review aims to discuss the use of gas chromatography (GC) methods as a tool for enantiomeric separations. The separation of enantiomers by GC methodologies has been performed using both direct and indirect methods, nevertheless, the selected methodology depends not only on the physical chemical properties of the target compounds but also on the field of application. Indeed, enantiomeric separation by GC in the forensic toxicology field was frequently performed using indirect methods for pharmaceuticals and illicit drugs, while direct methods have been reported as the preferable choice for pesticides and polychlorinated biphenyl compounds. Concerning food analysis chiral separation have been mainly carried out by direct methods while for environmental analysis, both direct and indirect methods have been reported, depending on the class of compounds studied. This manuscript compiles analytical methods of enantioseparation by GC, both direct and indirect methods, and its applicability in forensic toxicology, food and environmental analysis.

摘要

如今，在制药、农用化学品、法医毒理学、食品和环境分析等不同领域，准确量化对映异构体的分析方法是必不可少的。本综述旨在讨论使用气相色谱 (GC) 方法作为对映体分离的工具。使用 GC 方法分离对映异构体已使用直接和间接方法进行，然而，所选方法不仅取决于目标化合物的物理化学性质，还取决于应用领域。事实上，在法医毒理学领域，GC 对映体分离经常使用间接方法对药物和非法药物进行，而直接方法已被报道为农药和多氯联苯化合物的首选方法。关于食品分析手性分离主要通过直接方法进行，而对于环境分析，根据所研究的化合物类别，直接和间接方法都有报道。这份手稿汇编了 GC 对映分离的分析方法，包括直接和间接方法，及其在法医毒理学、食品和环境分析中的适用性。