Abstract

Tannin acyl hydrolases catalyzes the hydrolysis of tannins and can be used in different industrial sectors such as in food, beverage, pharmaceutical and chemical industries. The aim of the current study was to assess tannase production by Serratia marcescens. The experiments showed activity variation between 0.25 and 59.05 U mL⁻¹ in Plackett-Burman design. The production was optimized after applying the Doehlert design, and an activity of 78.79 U mL⁻¹ was obtained, thus corresponding to an increase of 315.16 times. The enzyme had a peak activity at pH 6.0 and 40 °C. It was inhibited in the presence of metal ions such as Cu²⁺, Fe²⁺ and Ba²⁺ and was activated by Na¹⁺ and K¹⁺. Results indicate the feasibility of using tannase from S. marcescens in biotechnological processes, such as in beverages manufacturing and gallic acid production.

摘要

单宁酰基水解酶催化单宁水解，可用于食品、饮料、制药和化工等不同工业部门。当前研究的目的是评估粘质沙雷氏菌的单宁酶生产。实验显示Plackett-Burman 设计中的活性变化在 0.25 和 59.05 U mL ^-1之间。应用Doehlert设计优化产量，获得78.79 U mL ^-1的活性，相当于增加了315.16倍。该酶在 pH 6.0 和 40 °C 时具有峰值活性。在Cu ²⁺、Fe ²⁺和Ba ²⁺等金属离子存在下被抑制，被Na ¹⁺激活和 K ¹⁺。结果表明在生物技术过程中使用来自S. marcescens 的单宁酶的可行性，例如在饮料制造和没食子酸生产中。