Abstract Candida albicans biofilms are responsible for oral candidiasis. Fluoxetine is a widely used antidepressant, with certain anti-Candida activities. The antifungal activity of fluoxetine combined with various antifungals against C. albicans biofilms and oral candidiasis was evaluated in this study. The morphological change in the inhibition of fluoxetine on C. albicans biofilms was observed using SEM. The interactions between fluoxetine and antifungals against C. albicans biofilms were evaluated using microdilution checkerboard methods, FICI and the ΔE model. The synergistic combination was tested in vivo on the mice model of oral candidiasis. SEM imaging showed fluoxetine inhibited hyphal growth and biofilm formation. Fluoxetine combined with caspofungin exhibited synergistic effects against C. albicans biofilms. Antagonistic effects occurred when fluoxetine was combined with amphotericin B or terbinafine. Further, the fluoxetine combined with caspofungin significantly reduced the lesion score and CFU of C. albicans on the murine tongue (p < 0.05), and relieved oral candidiasis of the infected mice.

中文翻译：

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氟西汀联合抗真菌药对白色念珠菌生物膜和口腔念珠菌病的体外和体内抗真菌活性评价

摘要白色念珠菌生物膜负责口腔念珠菌病。氟西汀是一种广泛使用的抗抑郁药，具有一定的抗念珠菌活性。在这项研究中评估了氟西汀与各种抗真菌剂联合对抗白色念珠菌生物膜和口腔念珠菌病的抗真菌活性。使用 SEM 观察氟西汀对白色念珠菌生物膜的抑制作用的形态变化。使用微稀释棋盘法、FICI 和 ΔE 模型评估氟西汀和抗真菌剂对白色念珠菌生物膜的相互作用。在口腔念珠菌病小鼠模型上对协同组合进行了体内测试。SEM 成像显示氟西汀抑制菌丝生长和生物膜形成。氟西汀联合卡泊芬净对白色念珠菌生物膜表现出协同作用。氟西汀与两性霉素 B 或特比萘芬合用时会产生拮抗作用。此外，氟西汀联合卡泊芬净显着降低了小鼠舌头上白色念珠菌的病变评分和 CFU（p < 0.05），并缓解了受感染小鼠的口腔念珠菌病。