Frontiers in Cellular and Infection Microbiology ( IF 4.6 ) Pub Date : 2020-05-20 , DOI: 10.3389/fcimb.2020.00303 David Pacha-Herrera 1 , Gabriela Vasco 1, 2 , Cecilia Cruz-Betancourt 3 , Juan Miguel Galarza 3 , Verónica Barragán 1 , António Machado 1
Pregnancy outcomes and women's health are directly affected by vaginal microbiota. This microbiota consists of a dynamic ecosystem of various microbes in different ratios, which in healthy conditions protect the vaginal epithelium from infections. However, cases of vaginal infection are regularly diagnosed in women of reproductive age, contributing to more severe outcomes. Therefore, our main goal was to determine the prevalence of bacterial vaginosis (BV), aerobic vaginitis (AV), and vulvovaginal candidiasis (VVC) among Ecuadorian pregnant and non-pregnant women. A cross-sectional study was conducted among 217 women between 13 and 40 years old seeking primary healthcare in Carlos Andrade Marin Hospital (HCAM), Gynecological-Obstetric Hospital Isidro Ayora (HGOIA) and Center for Teaching Health Cipriana Dueñas during October 2018 to February 2019. The classical characterization of the vaginal microbiota was performed through microscopy by the Nugent criteria to evaluate the presence of BV, healthy and intermediate microbiota, by the criteria of Donders to determine the presence of AV and by the Marot-Leblond criteria to diagnose VVC. DNA extraction from vaginal samples and Polymerase Chain Reaction (PCR) analysis was performed to characterize the presence of
中文翻译:
通过 qPCR 对孕妇和非孕妇进行阴道微生物群评估和乳酸杆菌定量:一项试点研究。
妊娠结局和女性健康直接受到阴道微生物群的影响。该微生物群由不同比例的各种微生物组成的动态生态系统组成,在健康条件下可保护阴道上皮免受感染。然而,育龄妇女经常诊断出阴道感染病例,从而导致更严重的后果。因此,我们的主要目标是确定厄瓜多尔孕妇和非孕妇中细菌性阴道病 (BV)、需氧性阴道炎 (AV) 和外阴阴道念珠菌病 (VVC) 的患病率。2018 年 10 月至 2019 年 2 月期间,对 Carlos Andrade Marin 医院 (HCAM)、Isidro Ayora 妇产医院 (HGOIA) 和 Cipriana Dueñas 健康教学中心寻求初级保健的 217 名 13 至 40 岁女性进行了一项横断面研究阴道微生物群的经典表征是通过显微镜按照 Nugent 标准评估 BV、健康和中间微生物群的存在,按照 Donders 标准确定 AV 的存在,并按照 Marot-Leblond 标准诊断 VVC。从阴道样本中提取 DNA 并进行聚合酶链反应 (PCR) 分析,以表征是否存在