Osteoporosis is an important skeletal disease characterized by bone weakness and high risk of fracture in postmenopausal women. Tea consumption is known to play an important role in the prevention or alleviation of osteoporosis. However, the therapeutic effects of aqueous extracts of white tea (WT) have not been evaluated in osteoporosis rat models. The aim of this study was to investigate the potential anti-osteoporotic role of WT in ovariectomized (OVX) rats. WT was given orally at 0.5% w/v doses for 12 weeks in OVX rats. Biochemical parameters in blood samples, bone tartrate-resistant acid phosphatase (TRAP), C-terminal telopeptide of type 1 collagen (CTX) and estradiol levels were evaluated. Bone mineral density and bone mineral content values were measured in the left femur. In addition to histopathological examination, osteolcalcin, osteopontin and TUNEL levels were determined. OVX group data demonstrated that bone loss occurred by thinning of the metaphyseal growth plates of the femur. Similarly, the levels of TRAP and CTX, markers of osteoclastic activity, were found to be high concurrently with a decrease in femoral bone mineral density. In addition, increased osteolcalcin and osteopontin levels were present in the metaphyseal growth zones. On the other hand, while TRAP and CTX levels were suppressed in the OVX-WT group, bone mineral content increased. In addition, TUNEL, osteocalcin and osteopontin positivity decreased in the right femoral metaphysis growth zones, proliferating zone and resting zone cells. These results showed that chronic WT consumption has a protective effect by reducing bone resorption in OVX-induced osteoporotic rats.

中文翻译：

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白茶通过抑制卵巢切除术诱导的骨质疏松模型大鼠的 TRAP/CTX 通路减少骨质流失

骨质疏松症是一种重要的骨骼疾病，其特征是绝经后妇女的骨无力和骨折风险高。众所周知，喝茶在预防或缓解骨质疏松症方面发挥着重要作用。然而，尚未在骨质疏松大鼠模型中评估白茶 (WT) 水提取物的治疗效果。本研究的目的是研究 WT 在卵巢切除 (OVX) 大鼠中的潜在抗骨质疏松作用。WT 在 OVX 大鼠中以 0.5% w/v 剂量口服给药 12 周。评估了血液样本中的生化参数、骨抗酒石酸酸性磷酸酶 (TRAP)、C 端 1 型胶原蛋白 (CTX) 端肽和雌二醇水平。在左股骨中测量骨矿物质密度和骨矿物质含量值。除了组织病理学检查外，骨钙素、测定骨桥蛋白和 TUNEL 水平。OVX 组数据表明骨丢失是由于股骨干骺端生长板变薄而发生的。类似地，发现破骨细胞活性的标志物 TRAP 和 CTX 的水平升高，同时股骨骨矿物质密度降低。此外，干骺端生长区中存在增加的骨钙蛋白和骨桥蛋白水平。另一方面，虽然 OVX-WT 组的 TRAP 和 CTX 水平受到抑制，但骨矿物质含量增加。此外，在右侧股骨干骺端生长区、增殖区和静止区细胞中，TUNEL、骨钙素和骨桥蛋白阳性率降低。这些结果表明，慢性 WT 消耗通过减少 OVX 诱导的骨质疏松大鼠的骨吸收具有保护作用。