DNA metabarcoding was utilized for a large‐scale, multiyear assessment of biodiversity in Malaise trap collections from the Bavarian Forest National Park (Germany, Bavaria). Principal component analysis of read count‐based biodiversities revealed clustering in concordance with whether collection sites were located inside or outside of the National Park. Jaccard distance matrices of the presences of barcode index numbers (BINs) at collection sites in the two survey years (2016 and 2018) were significantly correlated. Overall similar patterns in the presence of total arthropod BINs, as well as BINs belonging to four major arthropod orders across the study area, were observed in both survey years, and are also comparable with results of a previous study based on DNA barcoding of Sanger‐sequenced specimens. A custom reference sequence library was assembled from publicly available data to screen for pest or invasive arthropods among the specimens or from the preservative ethanol. A single 98.6% match to the invasive bark beetle Ips duplicatus was detected in an ethanol sample. This species has not previously been detected in the National Park.

中文翻译：

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用于国家公园生物多样性监测的 DNA 元条形码：筛选入侵和害虫物种。

DNA 元条形码被用于对来自巴伐利亚森林国家公园（德国，巴伐利亚）的 Malaise 陷阱收集物中的生物多样性进行大规模、多年的评估。基于读取计数的生物多样性的主成分分析显示聚类与收集地点是否位于国家公园内部或外部一致。在两个调查年（2016 年和 2018 年）中，收集地点存在条形码索引号 (BIN) 的 Jaccard 距离矩阵显着相关。在两个调查年中都观察到了存在总节肢动物 BIN 以及属于整个研究区域的四个主要节肢动物目的 BIN 的总体相似模式，并且也与之前基于 Sanger-DNA 条形码的研究结果相当。测序标本。自定义参考序列库是从公开可用的数据中组装出来的，以筛选标本中或防腐剂乙醇中的害虫或侵入性节肢动物。与侵入性树皮甲虫的单个 98.6% 匹配在乙醇样品中检测到Ips duplicatus。该物种以前从未在国家公园中发现过。