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Real‐time ALT and LDH activities determined in viable precision‐cut mouse liver slices using hyperpolarized [1‐13C]pyruvate—Implications for studies on biopsied liver tissues
NMR in Biomedicine ( IF 2.7 ) Pub Date : 2020-06-18 , DOI: 10.1002/nbm.4354
Naama Lev‐Cohain 1 , Gal Sapir 1 , Talia Harris 1 , Assad Azar 1 , Ayelet Gamliel 1 , Atara Nardi‐Schreiber 1 , Sivaranjan Uppala 1 , Jacob Sosna 1 , J. Moshe Gomori 1 , Rachel Katz‐Brull 1
Affiliation  

In the paper by Lev‐Cohain et al,1 the calculated activities of LDH and ALT reported should have been 4‐fold lower. This erratum corrects the text and supporting information errors that result from this mistake. These corrections do not affect any other result in the paper or any of the paper's conclusions.

  • The 2nd sentence in the last paragraph of the Results should read as:

On average, LDH activity and ALT activity were found to range between 0.29 and 1.04 nmole/s/g wet weight and between 0.65 and 2.42 nmole/s/g wet weight, respectively, and both correlated to the ATP level of the sample (Supporting Sections S8 and S9).

  • The 1st sentence of the 3rd paragraph in the discussion should read as:

[1‐13C]lactate production in liver slices was found here to range between 0.29 and 1.04 nmole/s/g wet weight.

  • The 1st sentence of the 4th paragraph in the discussion should read as:

The [1‐13C]alanine production rate determined here was 0.65 to 2.42 nmole/s/g wet weight.

  • The y‐axes of Figure S8 and Figure S9 (upper two right panels) are corrected below.

Corrected Figure S8.

image

Corrected Figure S9.

image



中文翻译:

使用超极化[1-13C]丙酮酸盐在可行的精密切割小鼠肝切片中测定实时ALT和LDH活性-对活检肝组织研究的意义

在Lev-Cohain等人的论文中,1所报道的LDH和ALT的计算活性应该低4倍。此错误纠正了由于该错误导致的文本和支持信息错误。这些更正不会影响本文中的任何其他结果或本文的任何结论。

  • 2.第二句的结果应该读作的最后一段:

平均而言,发现LDH活性和ALT活性分别在0.291.04 nmole / s / g湿重之间以及0.652.42 nmole / s / g湿重之间,并且都与样品的ATP水平相关(支持S8和S9节)。

  • 在1的3句话讨论应改为段落:

[1- 13 C]在肝切片乳酸产生在这里发现范围之间0.291.04纳摩尔/秒/克净重。

  • 在1的4句话讨论应如下段落:

的[1- 13 C]丙氨酸生产速率确定这里是0.652.42纳摩尔/秒/克净重。

  • 图S8和图S9的y轴(右上两个面板)在下面进行了校正。

更正了图S8。

图片

更正了图S9。

图片

更新日期:2020-08-04
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