For salmonid producers, a common threat is Flavobacterium psychrophilum . Recent advancements in bacterial coldwater disease (BCWD) management include the development of a live‐attenuated immersion vaccine that cross‐protects against an array of F. psychrophilum strains. Emerging family Flavobacteriaceae cases associated with clinical disease have been increasing, including pathogenic isolates of Flavobacterium spp. and Chryseobacterium spp. The cross‐protective ability of a live‐attenuated F. psychrophilum vaccine was determined against three virulent Flavobacteriaceae isolates. Juvenile rainbow trout were vaccinated, developed high F. psychrophilum‐specific antibody titres and were challenged with Chryseobacterium spp. isolates (S25 and T28), a Flavobacterium sp. (S21) isolate, a mixed combination of S21:S25:T28, and a standard virulent F. psychrophilum CSF259‐93 strain. Results demonstrated strong protection in the CSF259‐93 vaccinated group (relative per cent survival (RPS)=94.44%) when compared to the relevant CSF259‐93 controls (p  < .001). Protection was also observed for vaccinated fish challenged with the S21:S25:T28 mix (RPS = 85.18%; p  < .001). However, protection was not observed with the S21, S25 or T28 isolates alone. Analysis of whole‐cell lysates revealed differences in protein banding by SDS‐PAGE, but conserved antigenic regions by Western blot in S25 and T28. Results demonstrate that this live‐attenuated vaccine provided protection against mixed flavobacterial infection and suggest further benefits against flavobacteriosis.

中文翻译：

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减毒活黄杆菌浸入疫苗对新型黄杆菌的交叉保护。和Chryseobacterium spp。株。

对于鲑鱼生产者来说，常见的威胁是精神黄杆菌。细菌冷水疾病（BCWD）管理的最新进展包括开发了一种减毒活浸疫苗，该疫苗可交叉防御一系列嗜冷链球菌菌株。与临床疾病有关的新兴黄杆菌科病例增加，包括黄杆菌属的致病菌。和Chryseobacterium spp。确定了一种减毒活的嗜热链球菌疫苗对三种强毒黄杆菌科细菌的交叉保护能力。幼年虹鳟鱼已接种疫苗，发育良好F. psychrophilum特异性抗体滴度，并被Chryseobacterium spp攻击。分离株（S25和T28），黄杆菌属。（S21）分离物，S21：S25：T28和标准强毒性嗜冷链球菌CSF259-93菌株的混合组合。结果表明，与相关的CSF259-93对照相比，接种CSF259-93的组具有较强的保护作用（相对生存率（RPS）= 94.44％）（p  <.001）。还观察到了用S21：S25：T28混合物攻击的接种鱼的保护作用（RPS = 85.18％；p <.001）。但是，单独使用S21，S25或T28分离株未观察到保护作用。全细胞裂解物的分析显示，通过SDS-PAGE蛋白质带的差异，但通过Western blot在S25和T28中保守了抗原区域。结果表明，这种减毒活疫苗提供了针对混合黄细菌感染的保护作用，并表明了针对黄细菌病的进一步益处。